AP2A1 Antibody / Clathrin Adaptor Protein Antibody (RQ6543)

AP2A1 Antibody Knockout Validated WB. Western blot testing of AP2A1 expression in human HeLa wild-type (WT) and AP2A1 knockout (KO) cell lysates with AP2A1 Antibody at 0.5 ug/ml. Lane 1: HeLa WT lysate; Lane 2: HeLa AP2A1 KO lysate. A specific band was detected at approximately 100-108 kDa in wild-type cells and was eliminated in AP2A1 knockout cells, confirming antibody specificity. AP2A1 Antibody / Clathrin Adaptor Protein Antibody provides knockout validated detection of AP2A1 and supports studies of clathrin-mediated endocytosis, vesicle trafficking and receptor internalization pathways.

AP2A1 Antibody Human, Mouse and Rat WB. Western blot testing of human 293T, human SH-SY5Y, human K562, rat brain and mouse brain lysates with AP2A1 Antibody at 0.5 ug/ml. Lane 1: human 293T; Lane 2: human SH-SY5Y; Lane 3: human K562; Lane 4: rat brain; Lane 5: mouse brain. A specific band was detected at approximately 100-108 kDa, consistent with the predicted molecular weight of AP2A1. AP2A1 Antibody / Clathrin Adaptor Protein Antibody, validated using AP2A1 knockout cells, supports studies of clathrin-mediated endocytosis, vesicle trafficking and receptor internalization pathways.

AP2A1 Antibody Human Liver Cancer IHC. Immunohistochemistry staining of FFPE human liver cancer tissue with AP2A1 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-AP2A1 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. AP2A1 Antibody / Clathrin Adaptor Protein Antibody, validated using AP2A1 knockout cells, demonstrates cytoplasmic staining and supports studies of clathrin-mediated endocytosis, vesicle trafficking and receptor internalization pathways.

AP2A1 Antibody Human Prostate Cancer IHC. Immunohistochemistry staining of FFPE human prostatic acinar adenocarcinoma tissue with AP2A1 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-AP2A1 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. AP2A1 Antibody / Clathrin Adaptor Protein Antibody, validated using AP2A1 knockout cells, demonstrates cytoplasmic staining in prostate cancer cells and supports studies of clathrin-mediated endocytosis, vesicle trafficking and receptor internalization pathways.

AP2A1 Antibody Rat Brain IHC. Immunohistochemistry staining of FFPE rat brain tissue with AP2A1 Antibody at 2 ug/ml. HIER was performed using pH 8 EDTA buffer, followed by blocking with 10% goat serum. The tissue section was incubated with rabbit anti-AP2A1 antibody overnight at 4°C, followed by HRP-conjugated goat anti-rabbit IgG secondary antibody and DAB chromogen detection. AP2A1 Antibody / Clathrin Adaptor Protein Antibody, validated using AP2A1 knockout cells, demonstrates neuronal staining consistent with AP-2 adaptor complex expression and supports studies of clathrin-mediated endocytosis, vesicle trafficking and neuronal membrane trafficking pathways.

AP2A1 Antibody SH-SY5Y Cell FACS. Flow cytometry testing of fixed human SH-SY5Y neuroblastoma cells with AP2A1 Antibody at 1 ug/million cells. Cells were fixed with 4% paraformaldehyde, blocked with 10% goat serum and incubated with AP2A1 antibody, followed by DyLight 488-conjugated goat anti-rabbit IgG secondary antibody detection. Red: unstained cells; Green: isotype control; Blue: AP2A1 antibody. AP2A1 Antibody / Clathrin Adaptor Protein Antibody, validated using AP2A1 knockout cells, demonstrates increased fluorescence intensity consistent with AP2A1 expression and supports studies of clathrin-mediated endocytosis, vesicle trafficking and receptor internalization pathways.