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Home >> Antibodies >> Anti-EpCAM Antibody

Anti-EpCAM Antibody (F49189)

  Catalog No Formulation Size Price (USD)  
Image F49189-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F49189-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Anti-EpCAM antibody western blot analysis in 293 lysate. Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Anti-EpCAM antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P16422
Applications Western blot : 1:1000
Flow Cytometry : 1:10-1:50
Limitations This anti-EpCAM antibody is available for research use only.
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Description

EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas.

Application Notes

Titration of the anti-EpCAM antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 57-85 from the human protein was used as the immunogen for this anti-EpCAM antibody.

Storage

Aliquot the anti-EpCAM antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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