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Home >> Antibodies >> Anti-EGFR Antibody

Anti-EGFR Antibody [clone SPM622] (V2486)

  Catalog No Formulation Size Price (USD)  
Image V2486-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V2486-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V2486SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
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V2486IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC: Formalin-fixed, paraffin-embedded human placenta stained with anti-EGFR antibody (SPM622).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name SPM622
Purity Protein G affinity chromatography
UniProt P00533
Localization Cell surface
Applications Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT
Limitations This anti-EGFR antibody is available for research use only.
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Description

This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Application Notes

Optimal dilution of the anti-EGFR antibody should be determined by the researcher.

1. Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37oC
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Human EGFR purified from A431 cells was used as the immunogen for the anti-EGFR antibody.

Storage

Store the anti-EGFR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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