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Home >> Antibodies >> Anti-CK19 Antibody

Anti-CK19 Antibody [clone SPM266] (V9126)

  Catalog No Formulation Size Price (USD)  
Image V9126-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 459
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V9126-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 209
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V9126SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 459
IHC analysis of formalin-fixed, paraffin-embedded human endometrial carcinoma stained with anti-CK19 antibody (clone SPM266).
Western blot testing of human HepG2 lysate with anti-CK19 antibody (clone SPM266).
FACS staining of methanol-fixed human MCF7 cells with anti-CK19 antibody (red, clone SPM266) and isotype control (blue).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, lambda + IgG1, kappa
Clone Name SPM266
Purity Protein G affinity chromatography
UniProt P08727
Localization Cytoplasmic
Applications Flow cytometry : 1-2ug/10^6 cells
Immunofluorescence : 1-2ug/ml
Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This anti-CK19 antibody is available for research use only.
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Description

CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast.

Application Notes

The optimal dilution of the anti-CK19 antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

The human breast cancer cell line MCF-7 was used as the immunogen for this anti-CK19 antibody. Its epitope maps between amino acids 312-335.

Storage

Store the anti-CK19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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