AMBP Antibody Mouse Monoclonal AMBP/4533 / Bikunin [clone AMBP/4533] (V5300)

HuProt protein microarray analysis of AMBP Antibody Mouse Monoclonal AMBP/4533. Binding specificity was evaluated using a HuProt microarray containing more than 19,000 full-length human proteins. The antibody shows the strongest signal for Alpha-1-microglobulin-bikunin precursor (AMBP), with a Z-score of 105.55 and an S-score of 105.25, indicating strong target recognition and high relative specificity. Secondary signals for unrelated proteins such as Ubiquitin and STON2 show substantially lower Z-scores, supporting the preferential binding of this monoclonal antibody to its intended AMBP target.

SDS-PAGE analysis of purified, BSA-free AMBP antibody (clone AMBP/4533) as confirmation of integrity and purity.

Immunohistochemistry of AMBP Antibody Mouse Monoclonal AMBP/4533 in human ovarian cancer tissue. Formalin-fixed, paraffin-embedded human ovarian carcinoma sections demonstrate cytoplasmic HRP-DAB brown staining in tumor epithelial cells consistent with Alpha-1-microglobulin-bikunin precursor (AMBP) expression. Surrounding stromal components show comparatively weaker staining. The inset panel shows PBS used in place of primary antibody as a negative control, demonstrating minimal background signal. Heat-induced epitope retrieval was performed by boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 20 min followed by cooling prior to antibody incubation.