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Home >> Antibodies >> Alpha SNAP Antibody / NAPA / SNAPA

Alpha SNAP Antibody / NAPA / SNAPA (RQ8316)

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  Catalog No Formulation Size Price (USD)  
Image RQ8316 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of fixed and permeabilized human 293T cells with Alpha SNAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha SNAP antibody.
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Flow cytometry testing of fixed and permeabilized human 293T cells with Alpha SNAP antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Alpha SNAP antibody.
Western blot testing of human 1) 293T, 2) MCF7, 3) A549, 4) K562, 5) ThP-1 and 6) PC-3 cell lysate with Alpha SNAP antibody. Predicted molecular weight ~33 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P54920
Applications Western blot : 0.5-1ug/ml
Flow cytometry : 1-3ug/million cells
Limitations This Alpha SNAP antibody is available for research use only.
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Description

N-ethylmaleimide-sensitive factor Attachment Protein Alpha, also known as SNAP-alpha, is a SNAP protein that is involved in the intra-cellular trafficking and fusing of vesicles to target membranes in cells. This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene.

Application Notes

Optimal dilution of the Alpha SNAP antibody should be determined by the researcher.

Immunogen

Amino acids KEAEAMALLAEAERKVKNSQSFFC were used as the immunogen for the Alpha SNAP antibody.

Storage

After reconstitution, the Alpha SNAP antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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