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Home >> Antibodies >> ALOX12 Antibody

ALOX12 Antibody (F54466)

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  Catalog No Formulation Size Price (USD)  
Image F54466-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54466-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human skin tissue with ALOX12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human skin tissue with ALOX12 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Western blot testing of 1) human HeLa and 2) rat spleen lysate with ALOX12 antibody. Predicted molecular weight ~76 kDa.
Western blot testing of mouse lung lysate with ALOX12 antibody. Predicted molecular weight ~76 kDa.
Immunofluorescent staining of human A549 cells with ALOX12 antibody (green) and anti-Actin (red).
Flow cytometry testing of human K562 cells with ALOX12 antibody; Blue=isotype control, Green= ALOX12 antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P18054
Localization Cytoplasmic
Applications Western blot : 1:500-1:2000
Immunofluorescence : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Immunohistochemistry (FFPE) : 1:25
Limitations This ALOX12 antibody is available for research use only.
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Description

Oxygenase and 14,15-leukotriene A4 synthase activity.

Application Notes

The stated application concentrations are suggested starting points. Titration of the ALOX12 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 618-650 from the human protein was used as the immunogen for the ALOX12 antibody.

Storage

Aliquot the ALOX12 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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