ALOX12 Antibody / Lipid Peroxidation Enzyme Antibody (F54466)

ALOX12 Antibody Human Skin IHC. Immunohistochemical staining of FFPE human skin tissue with ALOX12 antibody. Heat-induced epitope retrieval (HIER) was performed by steaming tissue sections in pH 6 citrate buffer for 20 minutes prior to staining. Strong cytoplasmic immunoreactivity is observed throughout the epidermis, consistent with the expected expression of 12S-Lipoxygenase (ALOX12), a lipid peroxidation enzyme that catalyzes arachidonic acid metabolism and contributes to epidermal differentiation, skin barrier homeostasis, inflammatory signaling, and oxidative stress responses. Cell nuclei are counterstained with hematoxylin (blue).

ALOX12 Antibody Human HeLa and Rat Spleen WB. Western blot testing of 1) human HeLa and 2) rat spleen lysates with ALOX12 antibody. A distinct immunoreactive band is detected at approximately 76 kDa, consistent with the predicted molecular weight of 12S-Lipoxygenase (ALOX12). The results demonstrate cross-species detection of this lipid peroxidation enzyme, which catalyzes arachidonic acid metabolism to generate bioactive lipid mediators involved in platelet function, inflammatory signaling, oxidative stress, and cellular homeostasis.

ALOX12 Antibody Mouse Lung WB. Western blot testing of mouse lung tissue lysate with ALOX12 antibody. A distinct immunoreactive band is detected at approximately 76 kDa, consistent with the predicted molecular weight of 12S-Lipoxygenase (ALOX12). The results demonstrate specific detection of this lipid peroxidation enzyme, which catalyzes arachidonic acid metabolism to generate bioactive lipid mediators involved in inflammatory signaling, oxidative stress, lipid metabolism, and regulation of cellular responses.

ALOX12 Antibody A549 Cell IF. Immunofluorescent staining of human A549 cells with ALOX12 antibody (green) and anti-Actin antibody (red). ALOX12 immunoreactivity is observed predominantly within the cytoplasm in a punctate distribution, consistent with the expected localization of 12S-Lipoxygenase (ALOX12), a lipid peroxidation enzyme that catalyzes arachidonic acid metabolism and generates bioactive lipid mediators involved in inflammatory signaling, oxidative stress, ferroptosis, and regulation of cellular homeostasis. Actin (red) highlights the cytoskeletal network and cellular morphology.

ALOX12 Antibody K562 Cell FACS. Flow cytometric analysis of human K562 cells stained with ALOX12 antibody. Blue: isotype control. Green: ALOX12 antibody. The rightward shift of the antibody-stained population relative to the isotype control demonstrates specific detection of 12S-Lipoxygenase (ALOX12), a lipid peroxidation enzyme that catalyzes arachidonic acid metabolism and generates bioactive lipid mediators involved in inflammatory signaling, oxidative stress, ferroptosis, and regulation of cellular responses.