- Tel: 858.663.9055
-
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Allograft Inflammatory Factor 1 Antibody / AIF1 Cross-Species WB Antibody [clone AIF1/8970R] (V5150)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
V5150-100UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 100 ug | 559 | |
|
|
V5150-20UG | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide | 20 ug | 259 | |
|
|
V5150SAF-100UG | 1 mg/ml in 1X PBS; BSA free, sodium azide free | 100 ug | 559 |
| Availability | 1-3 business days |
| Species Reactivity | Human, Mouse, Rat, Rabbit, Hamster, Guinea pig |
| Format | Purified |
| Host | Rabbit |
| Clonality | Recombinant Rabbit Monoclonal |
| Isotype | Rabbit IgG, kappa |
| Clone Name | AIF1/8970R |
| Purity | Protein A/G affinity |
| UniProt | P55008 |
| Localization | Cytoplasm, Cell Surface |
| Applications | Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT (human) Western Blot : 2-4ug/ml (Human/Mouse/Rat/Rabbit/Hamster/Guinea pig) |
| Limitations | This Allograft Inflammatory Factor 1 Antibody / AIF1 Cross-Species WB Antibody is available for research use only. |
|
Review this product on BioCompare and get a $20 Amazon gift card
|
Related Products
|
Allograft inflammatory factor 1 (AIF1) is a calcium-binding cytoplasmic protein encoded by the AIF1 gene and expressed in macrophages and microglia, where it functions as a conserved marker of immune cell activation across multiple species. Allograft Inflammatory Factor 1 Antibody / AIF1 Cross-Species WB Antibody (clone AIF1/8970R) targets this protein and is well suited for detecting AIF1 expression by western blot in diverse biological samples. AIF1 antibody, also referred to as IBA1 antibody and Allograft inflammatory factor 1 antibody in the literature, recognizes a key regulator of cytoskeletal dynamics and inflammatory signaling in monocyte-derived cells.
Functionally, AIF1 plays a central role in immune cell activation and motility. It participates in actin-bundling processes that enable macrophages and microglia to migrate, change morphology, and respond to inflammatory cues. AIF1 expression is upregulated in activated immune cells and is associated with processes such as phagocytosis, cytokine production, and tissue surveillance. These functions make AIF1 a widely used marker for studying inflammation and immune responses across different experimental systems.
One of the defining features of AIF1 is its high degree of conservation across mammalian species, allowing for consistent detection in human, mouse, rat, and other model organisms. This conservation supports its use in cross-species western blot applications, where a single antibody can detect AIF1 protein in multiple tissue types, including brain, kidney, and spleen. In these tissues, AIF1 is typically observed as a cytoplasmic protein associated with macrophage and microglial populations, reflecting its role in immune cell function.
Structurally, AIF1 contains EF-hand calcium-binding domains that regulate its interaction with actin filaments and intracellular signaling pathways. These domains enable calcium-dependent modulation of cytoskeletal organization and cellular activation. AIF1 has been shown to co-localize with actin structures and membrane-associated regions, supporting its involvement in cell motility and immune responses. In addition to structural roles, AIF1 participates in signaling pathways that regulate inflammatory gene expression and immune cell activation states.
Altered AIF1 expression is associated with a range of pathological conditions, including inflammatory diseases, transplant rejection, and tumor-associated macrophage activity. In the central nervous system, increased AIF1 expression is a hallmark of microglial activation in neurodegenerative conditions, while in peripheral tissues it reflects macrophage-driven inflammatory responses. These properties highlight AIF1 as a valuable marker for studying immune activation across multiple biological contexts.
For researchers requiring robust detection across species and sample types, this antibody provides reliable performance in western blot applications, supporting consistent identification of AIF1 / IBA1 protein in multi-species experimental systems. An AIF1 antibody is suitable for detecting this immune cell marker in studies of inflammation, immune signaling, and cross-species protein expression analysis.
For detection of AIF1 as a microglia marker across tissue types, including protein microarray validated performance, see our IBA1 antibody.
Optimal dilution of the Allograft Inflammatory Factor 1 Antibody / AIF1 Cross-Species WB Antibody should be determined by the researcher.
A recombinant partial protein sequence (within amino acids 1-146) from the human protein was used as the immunogen for the Allograft Inflammatory Factor 1 antibody.
Aliquot the Allograft Inflammatory Factor 1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
AIF1 antibody, IBA1 antibody, Allograft inflammatory factor 1 antibody, AIF1 western blot antibody, IBA1 WB antibody
Your bulk quote request has been submitted successfully!
Please contact us if you have any questions.
Powered by Bioz
