Aldose Reductase Antibody / Polyol Pathway Marker [clone CPTC-AKR1B1-3] (V7403)

Aldose Reductase Antibody A549 IF. Immunofluorescence analysis of human A549 cells stained with Aldose reductase Antibody clone CPTC-AKR1B1-3 (green) and RedDot nuclear counterstain (red). Strong cytoplasmic fluorescence is observed throughout A549 cells, consistent with the expected intracellular localization of Aldose reductase / AKR1B1, an aldo-keto reductase family enzyme involved in polyol pathway metabolism, glucose-responsive signaling, and oxidative stress-associated cellular regulatory mechanisms.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using Aldose reductase antibody (clone CPTC-AKR1B1-3). These results demonstrate the foremost specificity of the CPTC-AKR1B1-3 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Aldose Reductase Antibody Prostate Cancer IHC. Immunohistochemistry analysis of FFPE human prostate cancer tissue stained with Aldose reductase antibody clone CPTC-AKR1B1-3. Strong cytoplasmic HRP-DAB brown staining is observed within prostate tumor epithelial cells, consistent with the expected intracellular localization of Aldose reductase / AKR1B1, a polyol pathway-associated metabolic enzyme involved in glucose metabolism and oxidative stress-responsive signaling pathways. Tissue sections underwent HIER using 10mM citrate buffer, pH 6, prior to immunostaining.

Aldose Reductase Antibody Multi-Sample WB. Western blot analysis of human kidney tissue and human HepG2, HEK293, HeLa, and Jurkat cell lysates using Aldose Reductase Antibody clone CPTC-AKR1B1-3. A predominant band is detected near approximately 35-40 kDa across multiple samples, consistent with the expected molecular weight range of Aldose reductase / AKR1B1, a cytoplasmic aldo-keto reductase family enzyme involved in polyol pathway metabolism, glucose-responsive signaling, and oxidative stress-associated cellular regulatory mechanisms.

Aldose Reductase Antibody Tissue and Cell Line WB. Western blot analysis of human kidney tissue together with HEK293 and HeLa cell lysates using Aldose reductase Antibody clone CPTC-AKR1B1-3. A strong band is detected near approximately 35-40 kDa across all samples, consistent with the expected molecular weight range of Aldose reductase / AKR1B1, a cytoplasmic aldo-keto reductase family enzyme involved in polyol pathway metabolism, oxidative stress regulation, and glucose-responsive cellular signaling pathways.

Aldose Reductase Antibody Cross-Species WB. Western blot analysis of human, mouse, rat, hamster, guinea pig, and rabbit kidney tissue lysates using Aldose reductase Antibody clone CPTC-AKR1B1-3. A distinct band is detected near approximately 35-40 kDa in human kidney tissue, consistent with the expected molecular weight range of Aldose reductase / AKR1B1, a polyol pathway-associated metabolic enzyme involved in glucose metabolism and oxidative stress-responsive cellular regulation. Minimal signal is observed in the additional species tested under these experimental conditions.