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Home >> Antibodies >> ALDH1A1 Antibody

ALDH1A1 Antibody (F44049)

  Catalog No Formulation Size Price (USD)  
Image F44049-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 439
Image
F44049-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 205
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Western blot analysis of ALDH1A1 antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ALDH1A1 gene (2).
ALDH1A1 Antibody Hepatocarcinoma IHC. Immunohistochemistry analysis of FFPE human hepatocarcinoma tissue stained with ALDH1A1 antibody.
Immunofluorescence analysis of anti-ALDH1A1 antibody and HeLa cells. Primary Ab was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow cytometric analysis of HepG2 cells using ALDH1A1 antibody (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Availability 1-3 business days
Species Reactivity Human
Predicted Reactivity Mouse, Primate
Format Purified
Host Rabbit
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Purified
UniProt P00352
Applications Western Blot : 1:1000
IHC (Paraffin) : 1:10-1:50
Immunofluorescence : 1:10-1:50
Flow Cytometry : 1:10-1:50
Limitations This ALDH1A1 antibody is available for research use only.
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Description

ALDH1A1 binds free retinal and cellular retinol-binding protein- bound retinal. Can convert/oxidize retinaldehyde to retinoic acid (By similarity). [UniProt]

For broader multi-application ALDH1A1 detection including IF and flow cytometry workflows, see our ALDH1A1 Antibody / Cancer Stem Cell and Retinoic Acid Metabolism Marker page.

Application Notes

Titration of the ALDH1A1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 302-331 from the human protein was used as the immunogen for this ALDH1A1 antibody.

Storage

Aliquot the ALDH1A1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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