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Home >> Antibodies >> ALAS1 Antibody / 5'-Aminolevulinate synthase 1

ALAS1 Antibody / 5'-Aminolevulinate synthase 1 [clone EOA-1] (RQ5401)

  Catalog No Formulation Size Price (USD)  
Image RQ5401 Antibody in PBS with 0.02% sodium azide and 50% glycerol 100 ug 359
Western blot testing of human HepG2 cell lysate with ALAS1 antibody. Predicted molecular weight ~71 kDa.
Availability 1-2 weeks
Species Reactivity Human
Format Purified
Clonality Rabbit Monoclonal
Isotype Rabbit IgG
Clone Name EOA-1
Purity Affinity purified
UniProt P13196
Applications Western blot : 1:500-1:2000
Immunofluorescence/Immunocytochemistry : 1:50-1:200
Limitations This ALAS1 antibody is available for research use only.
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Description

The ALAS1 gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate.

Application Notes

Optimal dilution of the ALAS1 antibody should be determined by the researcher.

Immunogen

A synthetic peptide specific to human ALAS1 was used as the immunogen for the ALAS1 antibody.

Storage

Store the ALAS1 antibody at -20oC.

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