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Home >> Antibodies >> AKT1 Antibody

AKT1 Antibody [clone RASTPK-1] (V3798)

  Catalog No Formulation Size Price (USD)  
Image V3798-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3798-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3798SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 429
IHC testing of FFPE human pancreas with AKT1 antibody (clone RASTPK-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2b, kappa
Clone Name RASTPK-1
Purity Protein G affinity chromatography
UniProt P31749
Localization Nucleus, plasma membrane, cytoplasm
Applications IHC (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This AKT1 antibody is available for research use only.
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Description

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. [UniProt]

Application Notes

The stated application concentrations are suggested starting points. Titration of the AKT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.

Storage

Store the AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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