AKR1B1 Antibody / Oxidative Stress Marker [clone CPTC-AKR1B1-2] (V3987)

AKR1B1 Antibody Prostate IHC. Immunohistochemistry analysis of FFPE human prostate tissue stained with AKR1B1 Antibody clone CPTC-AKR1B1-2. Strong cytoplasmic HRP-DAB brown staining is observed throughout glandular epithelial cells, consistent with the expected intracellular localization of Aldose reductase / AKR1B1, an oxidative stress-associated metabolic enzyme involved in reactive aldehyde detoxification, glucose-responsive signaling, and cellular redox regulatory pathways. Tissue sections underwent HIER using 10mM citrate buffer, pH 6, prior to immunostaining.

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AKR1B1 antibody (clone CPTC-AKR1B1-2). These results demonstrate the foremost specificity of the CPTC-AKR1B1-2 mAb.
Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free AKR1B1 antibody (clone CPTC-AKR1B1-2) as confirmation of integrity and purity.

AKR1B1 Antibody HeLa WB. Western blot analysis of human HeLa cell lysate using AKR1B1 Antibody clone CPTC-AKR1B1-2. A strong band is detected near approximately 35-40 kDa, consistent with the predicted molecular weight of Aldose reductase / AKR1B1, an oxidative stress-associated aldo-keto reductase family enzyme involved in reactive aldehyde detoxification, glucose-responsive signaling, and cellular redox regulatory pathways.

AKR1B1 Antibody A549 IF. Immunofluorescence analysis of human A549 cells stained with AKR1B1 Antibody clone CPTC-AKR1B1-2 (green) and DAPI nuclear counterstain (blue). Strong cytoplasmic fluorescence is observed throughout A549 cells, consistent with the expected intracellular localization of Aldose reductase / AKR1B1, an oxidative stress-associated metabolic enzyme involved in reactive aldehyde detoxification, glucose-responsive signaling, and cellular redox regulatory pathways.

AKR1B1 Antibody HEK293 WB. Western blot analysis of human HEK293 cell lysate using AKR1B1 Antibody clone CPTC-AKR1B1-2. A strong band is detected near approximately 35-40 kDa, consistent with the predicted molecular weight of Aldose reductase / AKR1B1, an oxidative stress-associated aldo-keto reductase family enzyme involved in reactive aldehyde detoxification, glucose-responsive signaling, and cellular redox regulatory pathways.

AKR1B1 Antibody Cross-Species WB. Western blot analysis of human, mouse, rat, hamster, guinea pig, and rabbit kidney tissue lysates using AKR1B1 Antibody clone CPTC-AKR1B1-2. A distinct band is detected near approximately 35-40 kDa in human kidney tissue, consistent with the predicted molecular weight of Aldose reductase / AKR1B1, an oxidative stress-associated aldo-keto reductase family enzyme involved in reactive aldehyde detoxification, glucose-responsive signaling, and cellular redox regulatory pathways. Minimal signal is observed in the additional species tested under these experimental conditions.

AKR1B1 Antibody Tissue and Cell Line WB. Western blot analysis of human kidney tissue together with HEK293 and HeLa cell lysates using AKR1B1 Antibody clone CPTC-AKR1B1-2. Strong bands are detected near approximately 35-40 kDa across all samples, consistent with the predicted molecular weight of Aldose reductase / AKR1B1, an oxidative stress-associated aldo-keto reductase family enzyme involved in reactive aldehyde detoxification, glucose-responsive signaling, and cellular redox regulatory pathways.