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Home >> Antibodies >> AKR1B1 Antibody / Aldose reductase

AKR1B1 Antibody / Aldose reductase [clone ADRD1-1] (V3975)

  Catalog No Formulation Size Price (USD)  
Image V3975-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 429
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V3975-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 199
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V3975SAF-100UG 1 mg/ml in 1X PBS; sodium azide free 100 ug 429
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V3975IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 429
IHC testing of FFPE human prostate cancer with AKR1B1 antibody (clone ADRD1-1). Required HIER: boil tissue sections in 10mM citrate buffer, pH6, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human HeLa cell lysate with AKR1B1 antibody (clone ADRD1-1). Predicted molecular weight ~36 kDa.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name ADRD1-1
Purity Protein G affinity chromatography
UniProt P15121
Localization Cytoplasmic
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This AKR1B1 antibody is available for research use only.
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Description

The AKR1B1 gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [RefSeq]

Application Notes

The optimal dilution of the AKR1B1 antibody for each application should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

Recombinant protein was used as the immunogen for this AKR1B1 antibody.

Storage

Store the AKR1B1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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