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Home >> Antibodies >> Adenylate kinase 4 Antibody / AK4

Adenylate kinase 4 Antibody / AK4 (F54385)

  Catalog No Formulation Size Price (USD)  
Image F54385-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 379
Image
F54385-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 149
Western blot testing of human 1) A431, 2) HeLa, 3) HepG2, 4) MCF7, 5) Raji and 6) mouse kidney lysate with Adenylate kinase 4 antibody. Predicted molecular weight ~25 kDa.
IHC testing of FFPE human heart tissue with Adenylate kinase 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE human kidney tissue with Adenylate kinase 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
IHC testing of FFPE mouse kidney tissue with Adenylate kinase 4 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Immunofluorescent staining of fixed and permeabilized human HepG2 cells with Adenylate kinase 4 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt P27144
Localization Cytoplasmic
Applications Immunofluorescence : 1:25
Immunohistochemistry (FFPE) : 1:25
Western blot : 1:500-1:2000
Limitations This Adenylate kinase 4 antibody is available for research use only.
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Description

Involved in maintaining the homeostasis of cellular nucleotides by catalyzing the interconversion of nucleoside phosphates. Efficiently phosphorylates AMP and dAMP using ATP as phosphate donor, but phosphorylates only AMP when using GTP as phosphate donor. Also displays broad nucleoside diphosphate kinase activity.

Application Notes

The stated application concentrations are suggested starting points. Titration of the Adenylate kinase 4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 119-153 from the human protein was used as the immunogen for the Adenylate kinase 4 antibody.

Storage

Aliquot the Adenylate kinase 4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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