60 kDa Heat Shock Protein Antibody / HSP60 60 kDa Antibody (F54512)

60 kDa Heat Shock Protein Antibody / HSP60 60 kDa Antibody Multi-Species Cell Expression WB. Western blot testing of human Jurkat T-cell leukemia (lane 1), HeLa cervical cancer (lane 2), HepG2 hepatocellular carcinoma (lane 3), MCF7 breast cancer (lane 4), mouse NIH 3T3 fibroblast (lane 5), and rat PC-12 pheochromocytoma (lane 6) cell lysates with 60 kDa Heat Shock Protein Antibody shows a strong predominant band at approximately 60 kDa in all six samples. The observed migration agrees with the predicted molecular weight of HSP60 and demonstrates recognition across human, mouse, and rat samples.

60 kDa Heat Shock Protein Antibody in HepG2 Hepatocellular Carcinoma Cells IF. Immunofluorescence staining of fixed and permeabilized human HepG2 hepatocellular carcinoma cells with 60 kDa Heat Shock Protein Antibody shows a prominent punctate to reticular cytoplasmic signal (green), consistent with the predominantly mitochondrial distribution of HSP60. Actin staining (red) highlights the cytoskeleton, while DAPI (blue) identifies the nuclei and emphasizes the extranuclear localization of the HSP60 signal.

60 kDa Heat Shock Protein Antibody in Human Lung Adenocarcinoma IHC. Immunohistochemistry staining of FFPE human lung adenocarcinoma tissue with 60 kDa Heat Shock Protein Antibody shows prominent granular cytoplasmic staining within tumor cells, consistent with the predominantly mitochondrial localization of HSP60. Heat-induced epitope retrieval was performed by steaming tissue sections in pH 6 citrate buffer for 20 min followed by cooling prior to staining.

60 kDa Heat Shock Protein Antibody in HeLa Cervical Cancer Cells FACS. Flow cytometry testing of fixed and permeabilized human HeLa cervical cancer cells with 60 kDa Heat Shock Protein Antibody shows a clear rightward shift in fluorescence of the antibody-stained population (green) relative to the isotype control (blue), demonstrating detection of intracellular HSP60 under the tested conditions.