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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2471 to 2480
    Histone H4 (mono methyl K20) Antibody / HIST1H4A (clone 31H81)
    Catalog No : FY13242
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human Jurkat cell lysate using the Histone H4 (mono methyl K20) antibody at 1:3000 dilution for 1 hour at room temperature. Predicted molecular weight ~11 kDa.
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    Caspase-3 Antibody (clone 30C96)
    Catalog No : FY13246
    Applications : WB, IHC, IP
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Caspase 3 expression in (1) Jurkat cell lysate; (2) NIH/3T3 cell lysate; (3) rat brain lysate using CASP3 antibody. The predicted molecular weight of Caspase 3 is at ~32 kDa.
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    img img img img
    RPN1 Antibody / Ribophorin I (clone 32R32)
    Catalog No : FY13254
    Applications : WB, IHC, ICC, IF, FACS
    Reactivity : Human, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the RPN1 antibody at 1:3000 dilution for 1 hour at room temperature. A predominant band is detected at an approximately 68 kDa in all samples, consistent with the mature N-glycosylated ER membrane protein ribophorin I described in the literature. Additional weaker bands between roughly 50 and 60 kDa, likely represent truncated or processed RPN1 species generated during normal ER associated degradation rather than distinct full length isoforms.
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    MCM6 Antibody / Minichromosome maintenance complex component 6 (clone 30M83)
    Catalog No : FY13258
    Applications : WB, IHC, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of MCM6 expression in (1) human MCF7 cell lysate; (2) mouse NIH 3T3 cell lysate using MCM6 antibody. Expected molecular weight: 92-105 kDa.
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    SEPTIN8 Antibody / Septin 8 (clone 30S14)
    Catalog No : FY13263
    Applications : WB, IHC, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Septin 8 expression in (1) human HeLa cell lysate; (2) mouse NIH 3T3 cell lysate using SEPTIN8 antibody. Predicted molecular weight ~56 kDa.
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    Phospho-Caveolin 1 (pTyr14) Antibody / CAV1 (clone 32C49)
    Catalog No : FY13264
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    CD163 Antibody (clone 32C14)
    Catalog No : FY13266
    Applications : WB, IHC
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Phospho-PKA C (pThr197) Antibody / PRKACA/PRKACB/PRKACG (clone 32P41)
    Catalog No : FY13272
    Applications : WB, IHC
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of P-PRKA/C/G using anti-Phospho-PKA C (pThr197) antibody. Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human U251 whole cell lysates, Lane 4: human SIHA whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Phospho-PKA C (Thr197) antibody at 1:500 overnight at 4oC, then washed with TBS-10%%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using an ECL Plus Western Blotting Substrate. The predicted molecular weight of P-PRKA/C/G is ~40 kDa.
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    img img img
    CD116 Antibody / CSF2RA (clone 31C12)
    Catalog No : FY13285
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of CD116 in human 293 cell lysate using CD116 antibody. A predominant band is detected at an approximately 55-60 kDa, running above the predicted ~46-47 kDa size but consistent with the higher apparent molecular weight reported for the heavily N glycosylated receptor.
    img
    PWP1 Antibody / Periodic tryptophan protein 1 (clone 30P43)
    Catalog No : FY13286
    Applications : WB
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of PWP1 expression in human HepG2 cell lysate. A predominant band is detected at an approximately 75 kDa, running above the predicted ~56 kDa size but consistent with the higher apparent molecular weight expected for the highly modified nuclear ribosome biogenesis factor PWP1.
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