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Rabbit Monoclonal Antibody / Antibodies

Home >> Antibodies >> Rabbit Monoclonal Antibody / Antibodies

Rabbit Monoclonal Antibody / Antibodies

The rabbit monoclonal antibody is a relatively new addition to the research antibody portfolio of mouse/rat monoclonals, and rabbit/donkey polyclonals. Some of the advantages of a rabbit monoclonal antibody are:

  • increased sensitivity to mouse antigens
  • unlike with mouse mAbs, secondary antibody will not cross-react with mouse immunoglobulin in test samples
  • a general lower background
  • a general increase in affinity allowing for less rabbit monoclonal antibody to be used per test

    • NSJ Bioreagents expanding line of rabbit monoclonal antibodies includes:

    Found all antibodies, displaying 2421 to 2430
    IQGAP2 Antibody / IQ motif containing GTPase activating protein 2 (clone 29I69)
    Catalog No : FY13019
    Applications : WB, IHC
    Reactivity : Human, Mouse
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of IQGAP2 using anti-IQGAP2 antibody. IQGAP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-IQGAP2 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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    ICAM1 Antibody / CD54 (clone 32I16)
    Catalog No : FY13020
    Applications : WB, IHC
    Reactivity : Mouse
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of ICAM1 using anti-ICAM1 antibody. ICAM1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-ICAM1 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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    PSMC5 Antibody / 26S proteasome regulatory subunit 8 (clone 29P84)
    Catalog No : FY13021
    Applications : WB, IHC, ICC, IF, IP, FACS
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Immunoprecipitation analysis using the antibody at 1:50 dilution. Western blot at 1:1000 dilution. Predicted molecular weight ~46 kDa.
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    NDUFB4 Antibody / NADH dehydrogenase ubiquinone 1 beta subcomplex subunit 4 (clone 30N45)
    Catalog No : FY13023
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot analysis of NDUFB4 using anti-NDUFB4 antibody. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB4 antibody at 1:500 overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A specific band was detected for NDUFB4 at approximately 15 kDa. The expected molecular weight of NDUFB4 is ~15 kDa.
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    Histone H3 (acetyl K36) Antibody / HIST1H3A (clone 31H90)
    Catalog No : FY13025
    Applications : WB, IHC, ICC, IF
    Reactivity : Human, Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the Histone H3 (acetyl K36) antibody at 1:1000 dilution for 1 hour at room temperature.
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    RALA Antibody / Ras-related protein Ral-A (clone 31R04)
    Catalog No : FY13031
    Applications : WB, IHC
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot testing of human samples using the RALA antibody at 1:5000 dilution for 1 hour at room temperature. Predicted molecular weight ~24 kDa.
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    PRPF31 Antibody / Pre-mRNA processing factor 31 (clone 29P97)
    Catalog No : FY13040
    Applications : WB, ICC, IF, FACS
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of PRPF31 expression in human Jurkat cell lysate using the PRPF31 antibody. Predicted molecular weight ~55 kDa.
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    TPH2 Antibody / Tryptophan hydroxylase 2 (clone 31T01)
    Catalog No : FY13043
    Applications : WB, IHC, IF
    Reactivity : Mouse, Rat
    Format : Liquid
    Recrabbitmono
    Immunohistochemical staining of TPH2 using anti-TPH2 antibody. TPH2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-TPH2 antibody overnight at 4oC. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37oC. The tissue section was developed using an HRP secondary and DAB substrate.
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    PPP2R5E Antibody / Serine threonine protein phosphatase 2A regulatory subunit B epsilon (clone 30P58)
    Catalog No : FY13045
    Applications : WB, IHC, ICC, IF, IP
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Immunoprecipitation analysis using the antibody at 1:50 dilution. Western blot at 1:1000 dilution. Predicted molecular weight: 50-56 kDa.
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    SYNE3 Antibody / Nesprin 3 (clone 30S39)
    Catalog No : FY13047
    Applications : WB
    Reactivity : Human
    Format : Liquid
    Recrabbitmono
    Western blot analysis of Nesprin 3 expression in human 293 cell lysate using Nesprin 3 antibody. A single band is detected at ~100 kDa, running slightly below the ~112 kDa prediction. The migration is consistent with the reported nesprin-3 isoform pattern (long form ~108-112 kDa, short form ~95-105 kDa) and the faster apparent mobility of coiled-coil nesprins on SDS-PAGE.
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