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Home >> Antibodies >> VEGFR2 Antibody

VEGFR2 Antibody (R31095)

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  Catalog No Formulation Size Price (USD)  
Image R31095 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing with VEGFR2 antibody and SMMC-7721 lysate. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
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Western blot testing with VEGFR2 antibody and SMMC-7721 lysate. Predicted molecular weight: ~152 (immature), 180-200 kDa (intermediate) and 220-230 kDa (mature).
IHC-P: VEGFR2 antibody testing of human lung cancer tissue
ICC testing of VEGFR2 antibody and HeLa cells
IHC-F testing of VEGFR2 antibody and human placenta tissue
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt P35968
Localization Cytoplasmic and cell surface
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
Immunohistochemistry (Frozen) : 0.5-1ug/ml
Immunocytochemistry : 0.5-1ug/ml
Limitations This VEGFR2 antibody is available for research use only.
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Description

KDR (Kinase Insert Domain Receptor), also known as FLK1, VEGFR 2 or VEGFR2, is a VEGF receptor. KDR is the human gene encoding it. Sait et al.(1995) likewise corrected the assignment to chromosome 4q11-q12 to the same region occupied also by PDGFRA and KIT, thus indicating the location of a cluster of receptor tyrosine kinase genes. Vascular endothelial growth factor(VEGF) is the only mitogen that specifically acts on endothelial cells. Its expression is upregulated by hypoxia, and its cell-surface receptor, known as fetal liver kinase-1(Flk1) in mouse, is exclusively expressed in endothelial cells(Plate et al., 1993). Flk1 is the mouse homolog of KDR(Matthews et al., 1991).

Application Notes

The stated application concentrations are suggested starting points. Titration of the VEGFR2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

An amino acid sequence from the middle region of human VEGF Receptor 2 (HHIHWYWQLEEECANE) was used as the immunogen for this VEGFR2 antibody.

Storage

After reconstitution, the VEGFR2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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