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Home >> Antibodies >> PROX1 Antibody

PROX1 Antibody (F47897)

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  Catalog No Formulation Size Price (USD)  
Image F47897-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
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F47897-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: HepG2 lysate; 2: mouse liver lysate; 3: SH-SY5Y lysate; Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
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Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: HepG2 lysate; 2: mouse liver lysate; 3: SH-SY5Y lysate; Predicted molecular weight is 83 kDa, observed at 80-110 kDa.
Western blot testing of PROX1 antibody at 1:2000 dilution. Lane 1: SW620 lysate; 2: SH-SY5Y lysate
PROX1 antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
PROX1 antibody IHC analysis in formalin fixed and paraffin embedded human brain tissue.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt Q92786
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This PROX1 antibody is available for research use only.
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Description

Prospero homeobox protein 1 is a transcription factor involved in developmental processes such as cell fate determination, gene transcriptional regulation and progenitor cell regulation in a number of organs. Plays a critical role in embryonic development and functions as a key regulatory protein in neurogenesis and the development of the heart, eye lens, liver, pancreas and the lymphatic system. Involved in the regulation of the circadian rhythm. Represses: transcription of the retinoid-related orphan receptor RORG, transcriptional activator activity of RORA and RORG and the expression of RORA/G-target genes including core clock components: ARNTL/BMAL1, NPAS2 and CRY1 and metabolic genes: AVPR1A and ELOVL3. [UniProt]

Application Notes

Titration of the PROX1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 492-520 from the human protein was used as the immunogen for this PROX1 antibody.

Storage

Aliquot the PROX1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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