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Home >> Antibodies >> PKC alpha Antibody / aPKC

PKC alpha Antibody / aPKC [clone 3G11-G11-G11] (F54061)

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  Catalog No Formulation Size Price (USD)  
Image F54061-0.1ML In PBS with 50% glycerol and 0.03% ProClin 300 0.1 ml 399
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Western blot testing of 1) human C2C12, 2) monkey COS7 and 3) rat C6 cell lysates using PKC alpha antibody at 1:1000. Predicted molecular weight ~77 kDa.
Western blot testing of 1) human C2C12, 2) monkey COS7 and 3) rat C6 cell lysates using PKC alpha antibody at 1:1000. Predicted molecular weight ~77 kDa.
Availability 1-3 business days
Species Reactivity Rat, Primate
Predicted Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a
Clone Name 3G11-G11-G11
Purity Protein G affinity
UniProt P17252
Applications Western blot : 1:1000
Limitations This PKC alpha antibody is available for research use only.
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Description

This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Application Notes

The stated application concentrations are suggested starting points. Titration of the PKC alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A synthetic PKC alpha/aPKC peptide was used as the immunogen for the PKC alpha antibody.

Storage

Store the PKC alpha antibody at -20oC.

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