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Home >> Antibodies >> Phospholamban Antibody

Phospholamban Antibody (F51974)

  Catalog No Formulation Size Price (USD)  
Image F51974-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F51974-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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Phospholamban antibody flow cytometric analysis of Jurkat cells (green) compared to a negative control (blue). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.
Phospholamban antibody IHC analysis in formalin fixed and paraffin embedded mouse heart tissue.
Western blot analysis of PLB / Phospholamban antibody and Jurkat lysate. Predicted molecular weight: 6/12/18/24kDa (monomer/dimer/oligomers).
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity
UniProt P26678
Applications Western blot : 1:1000
IHC (Paraffin) : 1:50-1:100
Flow Cytometry : 1:10-1:50
Limitations This Phospholamban antibody is available for research use only.
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Description

PLB is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.

Application Notes

Titration of the Phospholamban antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 1-30 from the human protein was used as the immunogen for the Phospholamban antibody.

Storage

Aliquot the Phospholamban antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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