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Home >> Antibodies >> MAP1LC3B Antibody

MAP1LC3B Antibody (F54364)

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  Catalog No Formulation Size Price (USD)  
Image F54364-0.4ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.4 ml 399
Image
F54364-0.08ML In 1X PBS, pH 7.4, with 0.09% sodium azide 0.08 ml 185
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IHC testing of FFPE human brain tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
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IHC testing of FFPE human brain tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
IHC testing of FFPE human liver tissue with MAP1LC3B antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Western blot testing of human HepG2 cell lysate with MAP1LC3B antibody. Predicted molecular weight ~15 kDa.
Western blot testing of mouse NIH 3T3 cell lysate with MAP1LC3B antibody. Predicted molecular weight ~15 kDa.
Flow cytometry testing of fixed and permeabilized human HepG2 cells with MAP1LC3B antibody; Blue=isotype control, Green= MAP1LC3B antibody.
Availability 1-3 business days
Species Reactivity Human, Mouse
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity purified
UniProt Q9GZQ8
Applications Immunohistochemistry (FFPE) : 1:25
Flow cytometry : 1:25 (1x10e6 cells)
Western blot : 1:500-1:2000
Limitations This MAP1LC3B antibody is available for research use only.
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Description

The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

Application Notes

The stated application concentrations are suggested starting points. Titration of the MAP1LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Immunogen

A portion of amino acids 9-33 from the human protein was used as the immunogen for the MAP1LC3B antibody.

Storage

Aliquot the MAP1LC3B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

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