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Home >> Antibodies >> Lumican Antibody / LUM

Lumican Antibody / LUM (R32130)

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  Catalog No Formulation Size Price (USD)  
Image R32130 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of mouse 1) testis, 2) thymus and 3) NIH3T3 lysate with Lumican antibody. Expected/observed moleculer weight: ~40/60 kDa (unmodified/glycosylated). (Ref 1)
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Western blot testing of mouse 1) testis, 2) thymus and 3) NIH3T3 lysate with Lumican antibody. Expected/observed moleculer weight: ~40/60 kDa (unmodified/glycosylated). (Ref 1)
IHC testing of FFPE mouse brain with Lumican antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
Availability 1-3 business days
Species Reactivity Mouse
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide
UniProt P51885
Localization Cytoplasmic, secreted
Applications Western blot : 0.1-0.5ug/ml
Immunohistochemistry (FFPE) : 0.5-1ug/ml
ELISA : 0.1-0.5ug/ml (mouse protein tested); request BSA-free format for coating
Limitations This Lumican antibody is available for research use only.
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  • Applications : WB, IHC-P, Direct ELISA
    Reactivity : Human, Mouse, Rat

Description

Lumican, also known as LUM, is an extracellular matrix protein that, in humans, is encoded by the LUM gene on chromosome 12. Lumican is a major keratan sulfate proteoglycan of the cornea but is ubiquitously distributed in most mesenchymal tissues throughout the body. And Lumican is involved in collagen fibril organization and circumferential growth, corneal transparency, and epithelial cell migration and tissue repair. Corneal transparency is possible due to the exact alignment of collagen fibers by lumican (and keratocan) in the intrafibrillar space.

Application Notes

Optimal dilution of the Lumican antibody should be determined by the researcher.

Immunogen

Amino acids 68-338 of mouse Lumican were used as the immunogen for the Lumican antibody.

Storage

After reconstitution, the Lumican antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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