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Home >> Antibodies >> GCLM Antibody

GCLM Antibody [clone CPTC-GCLM-1] (V8219)

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  Catalog No Formulation Size Price (USD)  
Image V8219-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V8219-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V8219SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC staining of FFPE human tonsil with GCLM antibody (clone CPTC-GCLM-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human tonsil with GCLM antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human 1) A549, 2) A431 and 3) HepG2 cell lysate with GCLM antibody. Expected molecular weight ~31 kDa.
SDS-PAGE analysis of purified, BSA-free GCLM antibody as confirmation of integrity and purity.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG2a, kappa
Clone Name CPTC-GCLM-1
Purity Protein G affinity chromatography
UniProt P48507
Localization Cytoplasm
Applications Western blot : 1-2ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT
Limitations This GCLM antibody is available for research use only.
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Description

GCLM, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Application Notes

Optimal dilution of the GCLM antibody should be determined by the researcher.

Immunogen

Recombinant human full-length GCLM protein was used as the immunogen for the GCLM antibody.

Storage

Store the GCLM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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