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Home >> Antibodies >> Fibronectin Antibody

Fibronectin Antibody [clone SPM246] (V9021)

  Catalog No Formulation Size Price (USD)  
Image V9021-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V9021-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V9021SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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IHC: Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma stained with Fibronectin antibody (SPM246).
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name SPM246
Purity Protein G affinity chromatography
UniProt P02751
Localization Connective tissue matrix
Applications Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Limitations This Fibronectin antibody is available for research use only.
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Description

Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. This mAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this mAb localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating mAb indicating that it can be used to specifically target tumor vessels in vivo.

Application Notes

The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 minutes.

Immunogen

A T-lymphoma biopsy was used as the immunogen for this Fibronectin antibody.

Storage

Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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