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Home >> Antibodies >> Early growth response protein 1 Antibody / EGR1

Early growth response protein 1 Antibody / EGR1 (RQ6978)

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  Catalog No Formulation Size Price (USD)  
Image RQ6978 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Flow cytometry testing of human K562 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
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Flow cytometry testing of human K562 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
Flow cytometry testing of mouse RAW264.7 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
Flow cytometry testing of rat RH35 cells with Early growth response protein 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Early growth response protein 1 antibody.
Western blot testing of 1) human K562, 2) human MCF-7, 3) human U-937, 4) human Jurkat, 5) rat brain, 6) rat PC-12, 7) mouse brain and 8) mouse RAW264.7 cell lysate with Early growth response protein 1 antibody. Predicted molecular weight: 57 kDa (monomer), ~110 kDa (cytoplasmic dimer), ~75 kDa (nuclear dimer).
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt P18146
Applications Western blot : 0.5-1 ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This Early growth response protein 1 antibody is available for research use only.
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Description

Early growth response protein 1, also known as ZIF268 or NGFIA, is a protein that in humans is encoded by the EGR1 gene. The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. This gene is mapped to 5q31.2. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Studies suggest this is a cancer suppressor gene. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity.

Application Notes

Optimal dilution of the Early growth response protein 1 antibody should be determined by the researcher.

Immunogen

Recombinant human protein (amino acids A2-M38) was used as the immunogen for the Early growth response protein 1 antibody.

Storage

After reconstitution, the Early growth response protein 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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