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Home >> Antibodies >> CDC45 Antibody / PORC-PI-1

CDC45 Antibody / PORC-PI-1 (RQ4295)

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  Catalog No Formulation Size Price (USD)  
Image RQ4295 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of rat 1) testis, 2) thymus, 3) brain, 4) lung and mouse 5) testis, 6) thymus, 7) brain, 8) lung and 9) HEPA1-6 lysate with CDC45 antibody at 0.5ug/ml. Predicted molecular weight ~66 kDa.
Western blot testing of rat 1) testis, 2) thymus, 3) brain, 4) lung and mouse 5) testis, 6) thymus, 7) brain, 8) lung and 9) HEPA1-6 lysate with CDC45 antibody at 0.5ug/ml. Predicted molecular weight ~66 kDa.
Availability 1-3 business days
Species Reactivity Mouse, Rat
Predicted Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose and 0.025% sodium azide
UniProt O75419
Localization Cytoplasm, nucleus
Applications Western blot : 0.5-1ug/ml
Direct ELISA : 0.1-0.5ug/ml
Limitations This CDC45 antibody is available for research use only.
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Description

Cell division control protein 45 homolog was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Alternate splicing results in multiple transcript variants.

Application Notes

Optimal dilution of the CDC45 antibody should be determined by the researcher.

Immunogen

A recombinant human protein corresponding to amino acids E166-A431 was used as the immunogen for the CDC45 antibody.

Storage

After reconstitution, the CDC45 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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