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Home >> Antibodies >> CD58 Antibody / LFA3

CD58 Antibody / LFA3 (R32395)

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  Catalog No Formulation Size Price (USD)  
Image R32395 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Western blot testing of human 1) HeLa and 2) K562 cell lysate with CD58 antibody. Predicted molecular weight: 28/40-70 kDa (unmodified/glycosylated).
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Western blot testing of human 1) HeLa and 2) K562 cell lysate with CD58 antibody. Predicted molecular weight: 28/40-70 kDa (unmodified/glycosylated).
IHC testing of FFPE human tonsil with CD58 antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to testing.
Flow cytometry testing of human HeLa cells with CD58 antibody at 1ug/10^6 cells (cells blocked with goat sera); Red=cells alone, Green=isotype control, Blue=CD58 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity
Buffer Lyophilized from 1X PBS with 2.5% BSA, 0.025% sodium azide
UniProt P19256
Localization Cytoplasmic, cell surface
Applications Western blot : 0.5-1ug/ml
Immunohistochemistry (FFPE) : 1-2ug/ml
Flow cytometry : 1-3ug/10^6 cells
Limitations This CD58 antibody is available for research use only.
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Description

CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.

Application Notes

Optimal dilution of the CD58 antibody should be determined by the researcher.

Immunogen

Amino acids F29-R215 from the human protein were used as the immunogen for the CD58 antibody.

Storage

After reconstitution, the CD58 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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