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Home >> Antibodies >> CD15 Antibody

CD15 Antibody (V3203)

  Catalog No Formulation Size Price (USD)  
Image V3203-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V3203-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V3203SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
Bulk quote request
IHC testing of FFPE human Hodgkin's lymphoma with CD15 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
Availability 1-3 business days
Species Reactivity Human, Mouse, Rat
Format Purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Protein A affinity
UniProt P22083
Localization Cytoplasmic and cell surface
Applications Flow cytometry : 2-4ug/10^6 cells
Immunofluorescence : 2-4ug/ml
Immunohistochemistry (FFPE) : 5-10ug/ml for 30 min at RT
Limitations This CD15 antibody is available for research use only.
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Description

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

Application Notes

Optimal dilution of the CD15 antibody should be determined by the researcher.

Immunogen

Purified human neutrophils were used as the immunogen for the CD15 antibody.

Storage

Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

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