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Home >> Antibodies >> BMI1 Antibody

BMI1 Antibody [clone PGRF4-1] (V7401)

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  Catalog No Formulation Size Price (USD)  
Image V7401-100UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 100 ug 519
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V7401-20UG 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide 20 ug 229
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V7401SAF-100UG 1 mg/ml in 1X PBS; BSA free, sodium azide free 100 ug 519
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V7401IHC-7ML Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only* 7 ml 519
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Western blot testing of human 293T lysate with BMI1 antibody (clone PGRF4-1). Expected molecular weight: 37-43 kDa.
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Western blot testing of human 293T lysate with BMI1 antibody (clone PGRF4-1). Expected molecular weight: 37-43 kDa.
IHC testing of FFPE human colon carcinoma with BMI1 antibody (clone PGRF4-1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Availability 1-3 business days
Species Reactivity Human
Format Purified
Clonality Monoclonal (mouse origin)
Isotype Mouse IgG1, kappa
Clone Name PGRF4-1
Purity Protein G affinity chromatography
UniProt P35226
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 1-2ug/ml
Prediluted IHC only format : incubate for 30 min at RT (1)
Limitations This BMI1 antibody is available for research use only.
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Description

The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.

Application Notes

Optimal dilution of the BMI1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Immunogen

A portion of amino acids 142-326 from the human protein was used as the immunogen for the BMI1 antibody.

Storage

Store the BMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Related Products

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