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banner Search results for nrf2

NRF2 Antibody / Nuclear factor erythroid 2-related factor 2 / NFE2L2

Catalog No : FY13169
Applications : ELISA, FACS, WB
Reactivity : Human
Format : Lyophilized
Flow Cytometry analysis of human RT4 cells using anti-NRF2 antibody. Overlay histogram showing RT4 cells stained with (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NRF2 antibody (1 ug/million cells) for 30 min at 20oC. DyLight 488 conjugated goat anti-rabbit IgG (5-10 ug/million cells) was used as secondary antibody for 30 minutes at 20oC. Isotype control antibody (Green line) was rabbit IgG (1 ug/million cells) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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PMF1 Antibody / Polyamine modulated factor 1 (clone 30P63)

Catalog No : FY13197
Applications : WB, IP
Reactivity : Human, Mouse, Rat
Format : Liquid
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Western blot analysis of PMF1 expression in (1) human HeLa cell lysate; (2) mouse RAW264.7 cell lysate using PMF1 antibody. Predicted molecular weight ~23 kDa.
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NRF-2 Antibody / NRF2L2

Catalog No : RQ5744
Applications : WB, IHC-P, IF, FACS, Direct ELISA
Reactivity : Human
Format : Antigen affinity purified
IHC staining of FFPE human breast cancer with NRF-2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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KEAP1 Antibody

Catalog No : F40464
Applications : WB, IHC, ELISA
Reactivity : Human
Pred. Reactivity :Pig
Format : Antigen affinity purified
KEAP1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle.
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KEAP1 Antibody

Catalog No : R34041
Applications : WB, IHC, ELISA (peptide)
Reactivity : Human, Mouse
Pred. Reactivity :Cow, Dog, Pig, Rat
Format : Antigen affinity purified
Western blot testing of NIH3T3 lysate with KEAP1 antibody at 0.2ug/ml. Predicted molecular weight ~70 kDa.
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KEAP1 Antibody (clone 297CT6.1.6)

Catalog No : F40314
Applications : WB, ELISA
Reactivity : Human
Pred. Reactivity :Pig
Format : Purified
KEAP1 antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~70 kDa.
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NRF-2 Antibody / NRF2L2

Catalog No : R34465
Applications : WB, ELISA (peptide)
Reactivity : Human
Pred. Reactivity :Cow, Dog, Mouse, Pig, Rat
Format : Antigen affinity purified
Western blot testing of human lung lysate with NRF2 antibody at 0.3ug/ml
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KEAP1 Antibody

Catalog No : R32225
Applications : WB
Reactivity : Human
Format : Antigen affinity purified
Western blot testing of human 1) HeLa, 2) MCF7 and 3) SW620 lysate wtih KEAP1 antibody. Expected/observed molecular weight ~70 kDa.
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TXNRD1 Antibody / Thioredoxin reductase 1

Catalog No : FY13051
Applications : WB, ELISA
Reactivity : Human
Format : Lyophilized
Western blot analysis of TXNRD1 using anti-TXNRD1 antibody. Lane 1: human Jurkat whole cell lysates, Lane 2: human whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TXNRD1 antibody at 0.5 ug/ml overnight at 4oC, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal was developed using enhanced chemiluminescent. A strong band is detected at ~65 kDa, with a weaker band at ~60 kDa, while the theoretical molecular weight of TXNRD1 is ~71 kDa. This downward shift is commonly reported and reflects the migration properties of the cytosolic selenoprotein, which often runs ~5-10 kDa lower than predicted. The two bands likely correspond to the major TXNRD1 isoform (~65 kDa) and a C-terminally truncated variant (~60 kDa) lacking the selenocysteine-containing tail.
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