- Tel: 858.663.9055
Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
ZO-3 Antibody (R31079)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
 |
R31079 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 429 |
| Availability | 1-3 business days |
| Species Reactivity | Mouse, Rat |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit IgG |
| Purity | Antigen affinity |
| Buffer | Lyophilized from 1X PBS with 2.5% BSA and 0.025% sodium azide/thimerosal |
| UniProt | Q9QXY1 |
| Applications | Western blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 0.5-1ug/ml |
| Limitations | This ZO-3 antibody is available for research use only. |
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TJP3 (Tight junction protein 3), also called ZO-3 (Zona occludens 3), is a protein that in humans is encoded by the TJP3 gene. TJP3/ZO-3 is a member of the family of membrane-associated guanylate kinase-like proteins (MAGUK) that associate with intracellular junctions (Itoh et al., 1999). Haskins et al.(1998) showed that recombinant Tjp3 binds in vitro translated Tjp1 and binds directly to the cytoplasmic tail of occludin but no binding was detected between Tjp3 and Tjp2. Roh et al.(2002) determined that Tjp3 interacted with PATJ(INADL) and that both proteins colocalized to tight junctions in vivo. In vitro studies showed that the sixth and eighth PDZ domains of PATJ mediated interaction with the Tjp3 C-terminal domain and with Cldn1, respectively.
The stated application concentrations are suggested starting amounts. Titration of the ZO-3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of mouse Zonula occludens protein 3 (RARDVESSDDEGYDWGPATDL) was used as the immunogen for this ZO-3 antibody (100% rat homology).
After reconstitution, the ZO-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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