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Home >> Antibodies >> ZNRF3 Antibody / Zinc/RING finger protein 3

ZNRF3 Antibody / Zinc/RING finger protein 3 (RQ7436)

  Catalog No Formulation Size Price (USD)  
Image RQ7436 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 429
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Immunofluorescent staining of FFPE human U-2 OS cells with ZNRF3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) Caco-2, 2) A549 and 3) HaCaT cell lysate with ZNRF3 antibody. Predicted molecular weight ~101 kDa.
Flow cytometry testing of human PC-3 cells with ZNRF3 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= ZNRF3 antibody.
Availability 1-3 business days
Species Reactivity Human
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit IgG
Purity Antigen affinity purified
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q9ULT6
Applications Western blot : 0.5-1ug/ml
Immunofluorescence : 5ug/ml
Flow cytometry : 1-3ug/million cells
Direct ELISA : 0.1-0.5ug/ml
Limitations This ZNRF3 antibody is available for research use only.
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Description

Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in cellular protein metabolic process and negative regulation of Wnt signaling pathway. Is integral component of plasma membrane.

Application Notes

Optimal dilution of the ZNRF3 antibody should be determined by the researcher.

Immunogen

E. coli-derived recombinant human protein (amino acids R54-E913) was used as the immunogen for the ZNRF3 antibody.

Storage

After reconstitution, the ZNRF3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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