ZNF774 Antibody PCRP-ZNF774-3F7 / Zinc Finger Protein 774 Antibody [clone PCRP-ZNF774-3F7] (V4397)

ZNF774 Antibody PCRP-ZNF774-3F7 Detection in HeLa Human Cervical Cancer Cells FACS. Flow cytometry analysis of PFA-fixed human HeLa cervical cancer cells using ZNF774 Antibody, clone PCRP-ZNF774-3F7, followed by goat anti-mouse IgG-CF488. The antibody-stained population (blue) shows a clear rightward fluorescence shift relative to unstained cells (red), demonstrating intracellular detection of Zinc Finger Protein 774 in human HeLa cells under the tested conditions.

Purified ZNF774 Antibody PCRP-ZNF774-3F7 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free ZNF774 Antibody, clone PCRP-ZNF774-3F7, under non-reducing and reducing conditions. The intact antibody migrates predominantly near 150 kDa under non-reducing conditions, while reduction produces antibody heavy- and light-chain bands near 50 kDa and 25 kDa, respectively. The observed banding pattern supports the integrity and purity of the monoclonal Zinc Finger Protein 774 Antibody preparation.

Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF774 antibody (clone PCRP-ZNF774-3F7). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference ZNF774 Antibody PCRP-ZNF774-3F7 Protein Microarray Validated. Protein microarray analysis of ZNF774 Antibody, clone PCRP-ZNF774-3F7, using a HuProt array containing more than 19,000 full-length human proteins. The intended ZNF774 target produced the dominant signal, with a Z-score of 18.00 and an S-score of 13.84. The Z-score measures signal strength in standard deviations above the array mean, while the S-score measures the separation between the intended target and the next-highest protein signal. The high S-score provides proteome-scale evidence supporting the target selectivity of this Protein Microarray Validated antibody.