ZNF774 Antibody PCRP-ZNF774-3F7 Zinc Finger Protein 774 V4397

	Catalog No	Formulation	Size	Price (USD)
	V4397-100UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	100 ug	559
Formulation
	V4397-20UG	0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide	20 ug	259
Formulation
	V4397SAF-100UG	1 mg/ml in 1X PBS; BSA free, sodium azide free	100 ug	559
Formulation

ZNF774 Antibody PCRP-ZNF774-3F7 Detection in HeLa Cervical Cancer Cells FACS. Flow cytometry analysis of PFA-fixed human HeLa cervical cancer cells using ZNF774 Antibody, clone PCRP-ZNF774-3F7, followed by goat anti-mouse IgG-CF488. The antibody-stained population (blue) shows a strong rightward fluorescence shift relative to unstained cells (red), demonstrating detection of Zinc Finger Protein 774 in human HeLa cells.

Purified ZNF774 Antibody PCRP-ZNF774-3F7 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free ZNF774 Antibody, clone PCRP-ZNF774-3F7, with 2 ug loaded under reducing (R) and non-reducing (NR) conditions. Under reducing conditions, bands corresponding to antibody heavy and light chains are visible at approximately 50 and 25 kDa. Under non-reducing conditions, the intact antibody migrates predominantly near 150 kDa. The observed banding pattern supports the integrity and purity of the monoclonal antibody preparation.

ZNF774 Antibody PCRP-ZNF774-3F7 Protein Microarray Validated. Protein microarray analysis of ZNF774 Antibody, clone PCRP-ZNF774-3F7, using a HuProt array containing more than 19,000 full-length human proteins. ZNF774 produced the highest-ranked signal with a Z-score of 18.00 and an S-score of 13.84, demonstrating strong preferential recognition of the intended target. The Z-score represents signal strength in standard deviations above the mean of all signals generated on the array, while the S-score measures separation between the intended target and the next-highest ranked protein. These results support high target specificity of this Zinc Finger Protein 774 Antibody.

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ZNF774 Antibody PCRP-ZNF774-3F7 Detection in HeLa Cervical Cancer Cells FACS. Flow cytometry analysis of PFA-fixed human HeLa cervical cancer cells using ZNF774 Antibody, clone PCRP-ZNF774-3F7, followed by goat anti-mouse IgG-CF488. The antibody-stained population (blue) shows a strong rightward fluorescence shift relative to unstained cells (red), demonstrating detection of Zinc Finger Protein 774 in human HeLa cells.

Purified ZNF774 Antibody PCRP-ZNF774-3F7 SDS-PAGE. SDS-PAGE analysis of purified, BSA-free ZNF774 Antibody, clone PCRP-ZNF774-3F7, with 2 ug loaded under reducing (R) and non-reducing (NR) conditions. Under reducing conditions, bands corresponding to antibody heavy and light chains are visible at approximately 50 and 25 kDa. Under non-reducing conditions, the intact antibody migrates predominantly near 150 kDa. The observed banding pattern supports the integrity and purity of the monoclonal antibody preparation.

ZNF774 Antibody PCRP-ZNF774-3F7 Protein Microarray Validated. Protein microarray analysis of ZNF774 Antibody, clone PCRP-ZNF774-3F7, using a HuProt array containing more than 19,000 full-length human proteins. ZNF774 produced the highest-ranked signal with a Z-score of 18.00 and an S-score of 13.84, demonstrating strong preferential recognition of the intended target. The Z-score represents signal strength in standard deviations above the mean of all signals generated on the array, while the S-score measures separation between the intended target and the next-highest ranked protein. These results support high target specificity of this Zinc Finger Protein 774 Antibody.

Availability	1-3 business days
Species Reactivity	Human
Format	Purified
Host	Mouse
Clonality	Monoclonal (mouse origin)
Isotype	Mouse IgG
Clone Name	PCRP-ZNF774-3F7
Purity	Protein A/G affinity
UniProt	Q6NX45
Localization	Nucleus
Applications	ELISA (Order BSA-free Format For Coating) : Flow Cytometry : 0.5-2ug/million cells
Limitations	This ZNF774 Antibody PCRP-ZNF774-3F7 / Zinc Finger Protein 774 Antibody is available for research use only.
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Description

ZNF774 Antibody PCRP-ZNF774-3F7 / Zinc Finger Protein 774 Antibody recognizes ZNF774, a member of the Kruppel C2H2-type zinc finger protein family. Zinc finger proteins comprise a large group of DNA-binding proteins characterized by zinc-coordinating domains that can mediate interactions with nucleic acids and other cellular components. Human ZNF774 is a 483-amino-acid protein containing multiple C2H2-type zinc finger domains and is associated with the nuclear compartment.

ZNF774 is predicted to function in transcriptional regulation. Current Gene Ontology annotations associate the protein with RNA polymerase II-specific DNA-binding transcription factor activity and sequence-specific binding to cis-regulatory regions. These predicted molecular functions are consistent with a role for Zinc Finger Protein 774 in controlling gene expression through interactions with regulatory DNA sequences. ZNF774 is also predicted to participate in regulation of transcription by RNA polymerase II.

Compared with many extensively characterized transcription factors, the biological functions of ZNF774 remain relatively poorly defined. UniProt currently describes the protein as potentially involved in transcriptional regulation rather than assigning a detailed experimentally established pathway or cellular function. This limited characterization makes ZNF774 relevant to studies seeking to define the roles of less extensively investigated C2H2 zinc finger proteins in transcriptional control and cellular biology.

Clone PCRP-ZNF774-3F7 is a mouse monoclonal antibody generated using recombinant full-length human ZNF774 protein as the immunogen. The antibody is Protein Microarray Validated using a HuProt array containing more than 19,000 full-length human proteins. NSJ Bioreagents offers clone PCRP-ZNF774-3F7 for researchers investigating ZNF774 expression and zinc finger protein biology. A ZNF774 Antibody provides a clone-defined reagent for studying Zinc Finger Protein 774 and its potential involvement in transcriptional regulation.

Explore our Epigenetics Antibodies page for additional reagents targeting proteins involved in transcriptional regulation, chromatin biology, and epigenetic control of gene expression.

Application Notes

Optimal dilution of the ZNF774 Antibody PCRP-ZNF774-3F7 / Zinc Finger Protein 774 Antibody should be determined by the researcher.

Immunogen

Recombinant full-length human ZNF774 protein was used as the immunogen for the ZNF774 Antibody PCRP-ZNF774-3F7.

Storage

Aliquot the ZNF774 Antibody PCRP-ZNF774-3F7 and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Alternate Names

ZNF774 antibody, Zinc finger protein 774 antibody, Zinc finger protein 774 homolog antibody

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ZNF774 Antibody PCRP-ZNF774-3F7 / Zinc Finger Protein 774 Antibody [clone PCRP-ZNF774-3F7] (V4397)

Description

Application Notes

Immunogen

Storage

Alternate Names

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Area of Research

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Protocols

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