ZNF488 Antibody / Transcription Factor Marker [clone PCRP-ZNF488-2D8] (V4097)

ZNF488 Antibody Uterus IHC. Immunohistochemistry analysis of FFPE human uterus tissue using ZNF488 antibody. The mouse monoclonal antibody clone PCRP-ZNF488-2D8 shows nuclear staining in epithelial cells, consistent with Zinc finger protein 488 / ZNF488 localization as a transcription factor marker. Signal is observed within cell nuclei of glandular and stromal compartments, reflecting transcriptional regulatory activity, while background staining remains low. HIER: boil tissue sections in 10 mM Tris with 1 mM EDTA, pH 9, for 20 min followed by cooling prior to staining.

SDS-PAGE analysis of purified, BSA-free ZNF488 antibody (clone PCRP-ZNF488-2D8) as confirmation of integrity and purity.

ZNF488 Antibody HeLa FACS. Flow cytometry analysis of PFA-fixed human HeLa cells using ZNF488 antibody. The mouse monoclonal antibody clone PCRP-ZNF488-2D8, detected with goat anti-mouse IgG-CF488 (blue), shows a clear right-shifted population compared to unstained cells (red), indicating positive intracellular staining of Zinc finger protein 488 / ZNF488. This shift supports detection of this nuclear transcription factor following permeabilization, consistent with its role as a transcription factor marker in proliferative epithelial cells.

ZNF488 Antibody Microarray Specificity Validation. Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using ZNF488 Mouse Monoclonal (PCRP-ZNF488-2D8). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.