Zebrafish WNT8A Antibody (RZ1444)

Zebrafish WNT8 Antibody Brain IHC. Immunohistochemistry analysis of zebrafish brain tissue using anti-WNT8A antibody demonstrates distinct cytoplasmic staining within neural cell populations, consistent with expression of WNT8, a secreted signaling ligand that functions as an important regulator of the Wnt signaling pathway. WNT8 plays a critical role in embryonic patterning, axis formation, tissue specification, and developmental signaling during vertebrate development. The observed staining pattern is consistent with the established involvement of Wnt pathway components in neurodevelopmental processes and cellular communication within the nervous system. Heat-mediated antigen retrieval was performed in EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with 2 ug/ml primary antibody overnight at 4°C. Detection was performed using a peroxidase-conjugated goat anti-rabbit IgG secondary antibody followed by DAB chromogen development.

Zebrafish WNT8 Antibody WB. Western blot analysis of WNT8A using anti-WNT8A antibody demonstrates a prominent immunoreactive band at approximately 42 kDa in zebrafish head, whole female, whole male, and embryo tissue lysates, consistent with the expected molecular weight of WNT8, the zebrafish ortholog of human WNT8A. WNT8 is a secreted signaling ligand that functions as an important regulator of canonical Wnt signaling pathways controlling embryonic patterning, axis specification, tissue differentiation, and vertebrate development. The observed expression across both developmental and adult-associated tissue samples is consistent with the established role of WNT8 in developmental signaling and regulation of cell fate decisions. Western blot was performed using 0.5 ug/ml primary antibody. Predicted molecular weight: ~40 kDa.