Zebrafish Vitellogenin 2 Antibody / VTG2 Antibody (RZ1365)

Zebrafish Vitellogenin 2 / VTG2 Antibody Pancreas IHC. Immunohistochemistry staining of FFPE zebrafish pancreas tissue using Zebrafish Vitellogenin 2 Antibody demonstrates distinct membranous and cytoplasmic HRP-DAB brown staining within clustered pancreatic epithelial cell populations. The staining pattern is consistent with expression of Vitellogenin 2 (VTG2), a large lipid-binding yolk precursor protein involved in nutrient transport, reproductive physiology, and estrogen-responsive signaling pathways. The observed immunoreactivity highlights discrete cellular populations within the pancreatic tissue and is consistent with the broad physiological functions of vitellogenin family proteins in vertebrate metabolism, nutrient mobilization, and developmental biology. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish Vitellogenin 2 / VTG2 Antibody Skeletal Muscle IHC. Immunohistochemistry staining of FFPE zebrafish skeletal muscle tissue using Zebrafish Vitellogenin 2 Antibody demonstrates weak to moderate HRP-DAB brown staining associated with elongated muscle fibers and interstitial cellular elements. The staining pattern is consistent with expression of Vitellogenin 2 (VTG2), a lipid-binding yolk precursor protein involved in nutrient transport, reproductive physiology, and estrogen-responsive biological processes. Although vitellogenins are classically associated with hepatic synthesis and oocyte development, detection in additional tissue compartments may reflect broader physiological roles in nutrient mobilization and metabolic regulation. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish Vitellogenin 2 / VTG2 Antibody Liver IHC. Immunohistochemistry staining of FFPE zebrafish liver tissue using Zebrafish Vitellogenin 2 Antibody demonstrates widespread granular cytoplasmic HRP-DAB brown staining throughout hepatocyte populations. The staining pattern is consistent with expression of Vitellogenin 2 (VTG2), a major yolk precursor protein synthesized primarily in the liver under estrogen-responsive regulation and subsequently transported to developing oocytes. Hepatic localization is consistent with the established biological role of vitellogenins in nutrient transport, reproductive physiology, embryonic nutrition, and endocrine-regulated metabolic processes. The widespread hepatocellular staining observed in this section supports the liver as a principal site of VTG2 production in zebrafish. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish Vitellogenin 2 / VTG2 Antibody Ovary IHC. Immunohistochemistry staining of FFPE zebrafish ovary tissue using Zebrafish Vitellogenin 2 Antibody demonstrates distinct cytoplasmic HRP-DAB brown staining associated with developing oocyte-containing structures and surrounding ovarian tissue. The staining pattern is consistent with expression and accumulation of Vitellogenin 2 (VTG2), a major yolk precursor protein that provides essential lipids, amino acids, and nutrients required for oocyte maturation and early embryonic development. Ovarian localization is consistent with the established role of VTG2 in reproductive physiology, vitellogenesis, and estrogen-regulated nutrient transport during oogenesis. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.