Zebrafish Vimentin Antibody / VIM Antibody (RZ1478)

Zebrafish Vimentin Antibody / VIM Antibody Zebrafish Brain IHC. Immunohistochemical staining of paraffin-embedded zebrafish brain tissue using Zebrafish Vimentin Antibody / VIM Antibody. Vimentin expression is detected predominantly within the cytoplasm of neural support and mesenchymal-associated cells, consistent with its role as a type III intermediate filament protein involved in cytoskeletal organization, tissue development, and cellular remodeling. Heat-induced epitope retrieval was performed in EDTA buffer prior to staining. Tissue sections were blocked with 10% goat serum and incubated with the primary antibody (2 ug/ml) overnight at 4°C, followed by an HRP-conjugated goat anti-rabbit secondary antibody and DAB chromogen for visualization.

Zebrafish Vimentin Antibody / VIM Antibody Zebrafish Head WB. Western blot analysis of zebrafish head tissue lysate using Zebrafish Vimentin Antibody / VIM Antibody. Two prominent immunoreactive bands are detected at approximately 54 kDa, consistent with the expected molecular weight of vimentin. The closely migrating bands likely represent different post-translationally modified forms or isoforms of the endogenous protein. Proteins were separated under reducing conditions, transferred to a nitrocellulose membrane, and detected following incubation with the primary antibody (0.5 ug/ml) and an HRP-conjugated goat anti-rabbit secondary antibody using ECL detection.