Zebrafish VEGF Antibody / VEGFAB Antibody (RZ1332)

Zebrafish VEGF / VEGFAB Antibody Brain IHC. Immunohistochemistry staining of FFPE zebrafish brain tissue using Zebrafish VEGF Antibody demonstrates cytoplasmic and extracellular HRP-DAB brown staining within neural tissue compartments adjacent to densely cellular brain regions. The staining pattern is consistent with expression of Vascular Endothelial Growth Factor AB (VEGFAB), a secreted angiogenic growth factor that regulates endothelial cell signaling, blood vessel formation, vascular permeability, and tissue vascularization. Signal is observed within brain-associated tissue structures, reflecting the established role of VEGF family proteins in neurovascular development and maintenance of the vertebrate vascular system. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish VEGF / VEGFAB Antibody Heart IHC. Immunohistochemistry staining of FFPE zebrafish heart tissue using Zebrafish VEGF Antibody demonstrates cytoplasmic and extracellular HRP-DAB brown staining within myocardial and vascular-associated tissue compartments. The staining pattern is consistent with expression of Vascular Endothelial Growth Factor AB (VEGFAB), a secreted angiogenic growth factor that regulates endothelial cell signaling, blood vessel formation, vascular permeability, and cardiovascular development. Signal is observed in regions associated with cardiac tissue architecture and vascular support structures, reflecting the essential role of VEGF signaling in maintenance and development of the vertebrate circulatory system. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish VEGF / VEGFAB Antibody Kidney IHC. Immunohistochemistry staining of FFPE zebrafish kidney tissue using Zebrafish VEGF Antibody demonstrates strong cytoplasmic HRP-DAB brown staining within renal tubular epithelial structures and associated glandular compartments. The staining pattern is consistent with expression of Vascular Endothelial Growth Factor AB (VEGFAB), a secreted angiogenic growth factor that regulates endothelial cell signaling, vascular permeability, blood vessel formation, and tissue vascularization. Prominent staining within renal epithelial populations is consistent with the importance of VEGF signaling in maintenance of vascular networks, tissue perfusion, and organ homeostasis. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish VEGF / VEGFAB Antibody Angiogenesis WB. Western blot analysis of zebrafish head tissue lysate using Zebrafish VEGF Antibody demonstrates a distinct immunoreactive band at approximately 40 kDa corresponding to Vascular Endothelial Growth Factor AB (VEGFAB). VEGFAB is a secreted angiogenic growth factor that regulates endothelial cell signaling, blood vessel formation, vascular permeability, and cardiovascular development. The observed band migrates at a higher apparent molecular weight than the predicted size of VEGFAB (~26 kDa), which may reflect post-translational modification or alternative protein forms present in zebrafish tissue. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate. Predicted molecular weight: ~26 kDa; observed molecular weight: ~40 kDa.