Zebrafish TUBA1A Antibody / Alpha Tubulin (RZ1377)

Zebrafish TUBA1A Antibody Embryo IF. Immunofluorescent staining of paraffin-embedded zebrafish embryo tissue using Zebrafish TUBA1A Antibody / Alpha Tubulin Antibody demonstrates strong green fluorescence throughout developing neural and embryonic tissue structures, consistent with the widespread distribution of alpha tubulin-containing microtubule networks during embryogenesis. TUBA1A is a highly conserved cytoskeletal protein required for microtubule assembly, neuronal differentiation, intracellular transport, and tissue morphogenesis. The staining pattern highlights regions of active cellular organization and developmental growth, while DAPI (blue) counterstains cell nuclei. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 5 µg/ml primary antibody, followed by DyLight 488-conjugated goat anti-rabbit IgG secondary antibody at 1:500 dilution for 30 minutes at 37°C.

Zebrafish TUBA1A Antibody Spinal Cord IHC. Immunohistochemical staining of paraffin-embedded zebrafish spinal cord tissue using Zebrafish TUBA1A Antibody / Alpha Tubulin Antibody demonstrates distinct cytoplasmic HRP-DAB brown staining within spinal cord cellular populations and surrounding neural structures. The staining pattern is consistent with expression of Alpha Tubulin (TUBA1A), a highly conserved microtubule-associated protein required for cytoskeletal organization, neuronal differentiation, axonal growth, and intracellular transport. TUBA1A immunoreactivity highlights neural tissue architecture and cellular organization within the developing zebrafish spinal cord. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 2 µg/ml primary antibody, followed by peroxidase-conjugated goat anti-rabbit IgG secondary antibody for 30 minutes at 37°C. Signal was visualized using DAB chromogen.

Zebrafish TUBA1A Antibody Brain IHC. Immunohistochemical staining of paraffin-embedded zebrafish brain tissue using Zebrafish TUBA1A Antibody / Alpha Tubulin Antibody demonstrates prominent cytoplasmic HRP-DAB brown staining throughout neural tissue compartments. The staining pattern is consistent with expression of Alpha Tubulin (TUBA1A), a highly conserved microtubule-associated protein that plays essential roles in neuronal differentiation, axonal extension, intracellular transport, and nervous system development. TUBA1A immunoreactivity highlights neuronal architecture and microtubule-rich cellular processes within the zebrafish brain, supporting its utility as a marker of cytoskeletal organization during neurodevelopment. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 2 µg/ml primary antibody, followed by peroxidase-conjugated goat anti-rabbit IgG secondary antibody for 30 minutes at 37°C. Signal was visualized using DAB chromogen.

Zebrafish TUBA1A Antibody Western Blot. Western blot analysis of zebrafish head tissue lysate (lane 1) and zebrafish embryo tissue lysate (lane 2) using Zebrafish TUBA1A Antibody / Alpha Tubulin Antibody demonstrates a distinct band at approximately 50 kDa, consistent with the expected molecular weight of Alpha Tubulin (TUBA1A). TUBA1A is a highly conserved microtubule-associated protein that contributes to cytoskeletal organization, intracellular transport, neuronal differentiation, and embryonic development. Detection of the expected molecular weight band in both zebrafish head and embryo samples supports expression of TUBA1A in neural and developing tissues and demonstrates the utility of this antibody for western blot applications. Electrophoresis was performed using a 10% SDS-PAGE gel under reducing conditions with 30 µg of protein loaded per lane. Signal was visualized using HRP-conjugated secondary antibody and ECL detection.