Zebrafish TPM1 Antibody / Tropomyosin Alpha-1 Chain Antibody (RZ1416)

Zebrafish TPM1 Antibody Colon IHC. Immunohistochemical analysis of paraffin-embedded zebrafish colon tissue using Zebrafish TPM1 Antibody / Tropomyosin Alpha-1 Chain Antibody demonstrates strong HRP-DAB brown staining within the smooth muscle layers surrounding the intestinal wall. The staining pattern is consistent with expression of TPM1, an actin-binding protein that stabilizes actin filaments and regulates actin-myosin interactions required for muscle contraction. Positive immunoreactivity highlights the contractile apparatus of gastrointestinal smooth muscle and supports the essential role of TPM1 in muscle structure, force generation, and maintenance of tissue integrity. Heat-induced antigen retrieval was performed in EDTA buffer. Tissue sections were blocked with 10% goat serum and incubated with TPM1 antibody (2 ug/ml) overnight at 4°C, followed by Peroxidase Conjugated Goat Anti-rabbit IgG and DAB chromogen detection.

Zebrafish TPM1 Antibody Multi-Tissue WB. Western blot analysis of 1) zebrafish head tissue lysate, 2) whole female zebrafish lysate, and 3) whole male zebrafish lysate using Zebrafish TPM1 Antibody / Tropomyosin Alpha-1 Chain Antibody. Prominent immunoreactive bands are detected at approximately 36-40 kDa in all samples, consistent with expression of TPM1. Tropomyosin proteins are known to exist as multiple isoforms generated through alternative splicing and differential promoter usage, resulting in closely migrating bands that are commonly observed by western blot analysis. TPM1 functions as a key actin-binding protein that stabilizes actin filaments and regulates actin-myosin interactions required for muscle contraction, sarcomere organization, and cytoskeletal integrity. The observed expression pattern supports the widespread role of TPM1 in muscle structure and contractile function throughout zebrafish tissues. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions. Predicted molecular weight: ~32 kDa. Observed molecular weights: ~36-40 kDa.