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Home >> Antibodies >> Zebrafish Suz12 Antibody / Isoforms a & b

Zebrafish Suz12 Antibody / Isoforms a & b (RZ1054)

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  Catalog No Formulation Size Price (USD)  
Image RZ1054 0.5mg/ml if reconstituted with 0.2ml sterile DI water 100 ug 539
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Immunohistochemical analysis of Suz12a/b protein using Zebrafish Suz12 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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Immunohistochemical analysis of Suz12a/b protein using Zebrafish Suz12 antibody and paraffin-embedded zebrafish brain tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot analysis of Suz12a/b protein using Zebrafish Suz12 antibody and zebrafish head tissue lysate. The predicted molecular weight of Suz12 is ~75 kDa (isoform a) and ~78 kDa (isoform b), commonly observed at 83-95 kDa.
Immunohistochemical analysis of Suz12a/b protein using Zebrafish Suz12 antibody and paraffin-embedded zebrafish spinal cord tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Immunohistochemical analysis of Suz12a/b protein using Zebrafish Suz12 antibody and paraffin-embedded zebrafish kidney tissue. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Availability 2-3 weeks
Species Reactivity Zebrafish
Format Antigen affinity purified
Clonality Polyclonal (rabbit origin)
Isotype Rabbit Ig
Purity Antigen affinity chromatography
Buffer Lyophilized from 1X PBS with 2% Trehalose
UniProt Q6DC03 , B0R1D5
Localization Nuclear
Applications Immunohistochemistry (FFPE) : 2-5 ug/ml
Western Blot : 0.5-1ug/ml
Limitations This Zebrafish Suz12 antibody is available for research use only.
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Description

Predicted to enable chromatin DNA binding activity. Predicted to act upstream of or within chromatin organization. Predicted to be part of ESC/E(Z) complex and RSC-type complex. Predicted to be active in nucleus. Is expressed in gonad; midbrain hindbrain boundary; pharyngeal arch; and visual system. Human ortholog(s) of this gene implicated in renal Wilms' tumor. Orthologous to human SUZ12 (SUZ12 polycomb repressive complex 2 subunit).

Application Notes

Optimal dilution of the Zebrafish Suz12 antibody should be determined by the researcher.

Immunogen

An E.coli-derived zebrafish recombinant protein (amino acids K38-K448) was used as the immunogen for the Zebrafish Suz12 antibody. This antibody will detect the a and b isoforms.

Storage

After reconstitution, the Zebrafish Suz12 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

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