Zebrafish SLC6A4 Antibody / SLC6A4A Antibody (RZ1366)

Zebrafish SLC6A4 / SLC6A4A Antibody Brain IHC. Immunohistochemistry staining of FFPE zebrafish brain tissue using Zebrafish SLC6A4 Antibody demonstrates distinct cytoplasmic HRP-DAB brown staining within scattered neuronal cell populations and neuropil-associated structures. The staining pattern is consistent with expression of Solute Carrier Family 6 Member 4A (SLC6A4A), a serotonin transporter that regulates serotonergic neurotransmission through reuptake of extracellular serotonin at neuronal synapses. Signal is observed throughout neural tissue compartments associated with neuronal communication and serotonin-dependent signaling pathways. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish SLC6A4 / SLC6A4A Antibody Serotonergic Brain IHC. Immunohistochemistry staining of FFPE zebrafish brain tissue using Zebrafish SLC6A4 Antibody demonstrates widespread cytoplasmic and neurite-associated HRP-DAB brown staining throughout neural tissue compartments. Signal is observed within neuronal cell populations and surrounding neuropil-rich regions, consistent with expression of Solute Carrier Family 6 Member 4A (SLC6A4A), a serotonin transporter involved in the regulation of serotonergic neurotransmission through reuptake of extracellular serotonin. The staining pattern highlights neural structures associated with serotonin-dependent signaling pathways and neuronal communication. Heat mediated antigen retrieval was performed in EDTA buffer. Primary antibody was incubated overnight at 4°C followed by detection using a peroxidase-conjugated goat anti-rabbit secondary antibody and DAB chromogen.

Zebrafish SLC6A4 / SLC6A4A Antibody Serotonin Transporter WB. Western blot analysis of whole zebrafish tissue lysate using Zebrafish SLC6A4 Antibody demonstrates a distinct immunoreactive band at approximately 70-80 kDa, consistent with expression of Solute Carrier Family 6 Member 4A (SLC6A4A). SLC6A4A encodes a serotonin transporter that regulates serotonergic neurotransmission through the reuptake of extracellular serotonin at neuronal synapses. The observed band migrates near the predicted molecular weight of SLC6A4A (~70 kDa) and supports detection of the endogenous protein in zebrafish tissue. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions followed by transfer to a nitrocellulose membrane. Signal was detected using an HRP-conjugated secondary antibody and enhanced chemiluminescent substrate. Predicted molecular weight: ~70 kDa.