Zebrafish SLC4A1 Antibody / SLC4A1A Antibody (RZ1389)

Zebrafish SLC4A1 Antibody Gill IHC. Immunohistochemical staining of paraffin-embedded zebrafish gill tissue using Zebrafish SLC4A1 Antibody / SLC4A1A Antibody demonstrates strong HRP-DAB brown staining within gill filament-associated cells and erythrocyte-rich structures. The staining pattern is consistent with expression of SLC4A1A, a chloride/bicarbonate exchanger that contributes to ion transport, acid-base regulation, and erythrocyte physiology. As the zebrafish ortholog of human SLC4A1 (Band 3/AE1), SLC4A1A plays an important role in maintaining cellular ionic balance and supporting carbon dioxide transport. Expression within gill-associated tissues is consistent with the involvement of anion transport proteins in respiratory physiology, gas exchange, and regulation of the aquatic ionic environment. Heat-mediated antigen retrieval was performed in EDTA buffer prior to overnight primary antibody incubation and HRP-DAB visualization.

Zebrafish SLC4A1 Antibody Spleen IHC. Immunohistochemical staining of paraffin-embedded zebrafish spleen tissue using Zebrafish SLC4A1 Antibody / SLC4A1A Antibody demonstrates strong HRP-DAB brown staining throughout erythrocyte-rich cellular populations within splenic tissue. The staining pattern is consistent with expression of SLC4A1A, the zebrafish ortholog of human SLC4A1 (Band 3/AE1), a chloride/bicarbonate exchanger that plays a critical role in erythrocyte membrane function, carbon dioxide transport, and acid-base regulation. SLC4A1A is highly associated with erythroid cells and contributes to maintenance of red blood cell membrane integrity and physiologic ion transport. The prominent splenic staining is consistent with the role of the spleen as a major hematopoietic and blood cell-associated organ in teleost fish. Heat-mediated antigen retrieval was performed in EDTA buffer prior to overnight primary antibody incubation and HRP-DAB visualization.

Zebrafish SLC4A1 Antibody Kidney IHC. Immunohistochemical staining of paraffin-embedded zebrafish kidney tissue using Zebrafish SLC4A1 Antibody / SLC4A1A Antibody demonstrates strong HRP-DAB brown staining within erythrocyte-rich cellular populations distributed throughout the renal hematopoietic compartment. The staining pattern is consistent with expression of SLC4A1A, the zebrafish ortholog of human SLC4A1 (Band 3/AE1), a chloride/bicarbonate exchanger that plays an essential role in erythrocyte membrane function, carbon dioxide transport, and acid-base regulation. SLC4A1A is highly associated with erythroid cells and contributes to maintenance of red blood cell membrane integrity and physiologic ion transport. The prominent staining observed within blood cell-rich regions of the zebrafish kidney is consistent with the role of the teleost kidney as a major hematopoietic organ involved in erythropoiesis and blood cell maintenance. Heat-mediated antigen retrieval was performed in EDTA buffer prior to overnight primary antibody incubation and HRP-DAB visualization.

Zebrafish SLC4A1 Antibody WB. Western blot analysis of zebrafish head tissue lysate (lane 1), whole female zebrafish tissue lysate (lane 2), and whole male zebrafish tissue lysate (lane 3) using Zebrafish SLC4A1 Antibody / SLC4A1A Antibody demonstrates a prominent band at approximately 101 kDa, consistent with the expected molecular weight of full-length SLC4A1A. SLC4A1A is the zebrafish ortholog of human SLC4A1, a chloride/bicarbonate exchanger also known as Anion Exchange Protein 1 (AE1) or Band 3. This erythrocyte-associated membrane protein plays a critical role in carbon dioxide transport, acid-base regulation, and maintenance of cellular ionic balance through bicarbonate and chloride exchange across the plasma membrane. Detection of SLC4A1A in multiple zebrafish tissue preparations is consistent with its established role in erythroid cell physiology, membrane transport, and hematopoietic function. Additional lower molecular weight bands are observed between approximately 28-35 kDa and may represent processed or proteolytically cleaved SLC4A1A species, which have been reported for Band 3 family proteins, or other factors influencing electrophoretic migration. Electrophoresis was performed on a 10% SDS-PAGE gel under reducing conditions with 30 µg of protein loaded per lane, followed by nitrocellulose transfer and HRP-ECL detection.