Zebrafish Septin7 Antibody / Septin7a / Septin7b (RZ1306)

Description

Zebrafish Septin7 is a member of the septin family of GTP binding proteins that are involved in cytoskeletal organization, membrane dynamics, and cell division. Septins form hetero oligomeric complexes that act as scaffolds and diffusion barriers, contributing to processes such as cytokinesis, vesicle trafficking, and cell polarity. Septin7 plays a crucial structural role in septin filament assembly and is required for proper cell morphology and intracellular transport.

Zebrafish Septin7 is an ortholog of the human SEPTIN7 protein, with strong sequence and functional conservation across species. In zebrafish, Septin7 is encoded by 2 isoforms, Septin7a and Septin7b. Both isoforms share similar functions but may exhibit differences in expression patterns during development or in specific tissues. The presence of Septin7a and Septin7b in zebrafish provides a unique opportunity to study how septin isoforms cooperate or diverge in their roles within the cytoskeleton and signaling pathways.

Septin7 is particularly important during cellular processes that require cytoskeletal remodeling, such as neurite outgrowth, epithelial polarity, and germ cell development. Disruption of Septin7 function can lead to defects in cytokinesis, altered vesicle trafficking, and impaired cell signaling.

Antibodies against zebrafish Septin7 protein are useful for studying the organization of septin filaments and their interactions with actin and microtubule networks. These antibodies are valuable in applications such as western blot, immunohistochemistry, immunofluorescence, and ELISA. The conservation of Septin7 between zebrafish and human systems also makes zebrafish a powerful model for exploring the fundamental roles of SEPTIN7 in cell division, morphogenesis, and disease.

Application Notes

Optimal dilution of the Zebrafish Septin7 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Septin7 recombinant protein (amino acids M1-K348) was used as the immunogen for the Zebrafish Septin7 antibody.

Storage

After reconstitution, the Zebrafish Septin7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.