Zebrafish RPL8 Antibody / Ribosomal Protein L8 Antibody (RZ1380)

Zebrafish RPL8 Antibody Pancreas IHC. Immunohistochemical staining of paraffin-embedded zebrafish pancreas tissue using Zebrafish RPL8 Antibody / Ribosomal Protein L8 Antibody demonstrates strong cytoplasmic HRP-DAB brown staining throughout pancreatic epithelial and secretory cell populations. The staining pattern is consistent with expression of RPL8, a structural component of the 60S large ribosomal subunit that participates in protein synthesis and ribosome function. Robust cytoplasmic localization reflects the abundance of ribosomes within metabolically active pancreatic cells, supporting the essential role of RPL8 in translational activity, cellular growth, and maintenance of tissue function. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 2 µg/ml primary antibody, followed by peroxidase-conjugated goat anti-rabbit IgG for 30 minutes at 37°C. DAB was used as the chromogenic substrate.

Zebrafish RPL8 Antibody Colon IHC. Immunohistochemical staining of paraffin-embedded zebrafish colon tissue using Zebrafish RPL8 Antibody / Ribosomal Protein L8 Antibody demonstrates prominent cytoplasmic HRP-DAB brown staining within intestinal epithelial cell populations lining the colonic mucosa. The staining pattern is consistent with expression of RPL8, a structural component of the 60S large ribosomal subunit that supports protein synthesis and ribosome function. Strong cytoplasmic localization reflects the abundance of translational machinery within metabolically active epithelial cells, highlighting the essential role of RPL8 in cellular growth, protein production, and maintenance of intestinal tissue homeostasis. Heat-mediated antigen retrieval was performed in EDTA buffer. Sections were blocked with 10% goat serum and incubated overnight at 4°C with 2 µg/ml primary antibody, followed by peroxidase-conjugated goat anti-rabbit IgG for 30 minutes at 37°C. DAB was used as the chromogenic substrate.

Zebrafish RPL8 Antibody Embryo Western Blot. Western blot analysis of zebrafish embryo tissue lysate (lane 1) using Zebrafish RPL8 Antibody / Ribosomal Protein L8 Antibody demonstrates a distinct band at approximately 35 kDa, consistent with detection of RPL8. Ribosomal Protein L8 (RPL8) is a structural component of the 60S large ribosomal subunit that supports ribosome assembly, protein synthesis, and translational activity. As an essential component of the cellular protein production machinery, RPL8 contributes to embryonic growth, tissue development, and maintenance of cellular biosynthetic capacity. Detection of RPL8 in zebrafish embryo lysate is consistent with the high translational demands associated with embryogenesis and developmental gene expression programs. Electrophoresis was performed using a 10% SDS-PAGE gel under reducing conditions with 30 µg of protein loaded per lane. Signal was visualized using HRP-conjugated secondary antibody and ECL detection.