Zebrafish RAD51 Antibody / RAD51 Recombinase Antibody (RZ1450)

Zebrafish RAD51 Antibody Testis IHC. Immunohistochemical analysis of RAD51 expression using anti-RAD51 antibody in paraffin embedded zebrafish testis tissue. RAD51, also known as RAD51 Recombinase, is a central mediator of homologous recombination and DNA double strand break repair that plays an essential role in meiotic chromosome segregation and germ cell maintenance. Prominent staining is observed within seminiferous structures, consistent with the high DNA repair activity associated with spermatogenesis. Heat mediated antigen retrieval was performed in EDTA buffer prior to staining. Sections were blocked with 10 percent goat serum and incubated with RAD51 antibody at 2 ug/ml overnight at 4 degrees Celsius. Detection was achieved using a peroxidase conjugated goat anti rabbit IgG secondary antibody and DAB chromogen. These results support the utility of Zebrafish RAD51 Antibody for studies of meiosis, genome stability, reproductive biology, and DNA damage responses.

Zebrafish RAD51 Antibody Embryo WB. Western blot analysis of RAD51 expression using anti-RAD51 antibody. RAD51, also known as RAD51 Recombinase, is a key mediator of homologous recombination and DNA double strand break repair that maintains genomic stability during development. Lane 1: zebrafish embryo tissue lysate. A specific band was detected at approximately 38 kDa. Although the predicted molecular weight of RAD51 is approximately 26 kDa, slower migration has been reported and may reflect post translational modifications, oligomerization, or species specific differences in electrophoretic mobility. These results demonstrate expression of RAD51 in zebrafish embryos and support the utility of this antibody for studies of DNA repair, genome maintenance, and developmental biology.