- Tel: 858.663.9055
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Email: info@nsjbio.com
- Tel: 858.663.9055
- Email: info@nsjbio.com
Zebrafish Rab14 Antibody / Rab14l (RZ1297)
Email: info@nsjbio.com
| Catalog No | Formulation | Size | Price (USD) | ||
|---|---|---|---|---|---|
|
RZ1297 | 0.5mg/ml if reconstituted with 0.2ml sterile DI water | 100 ug | 539 |
| Availability | 2-3 weeks |
| Species Reactivity | Zebrafish |
| Format | Antigen affinity purified |
| Clonality | Polyclonal (rabbit origin) |
| Isotype | Rabbit Ig |
| Purity | Antigen affinity chromatography |
| Buffer | Lyophilized from 1X PBS with 2% Trehalose |
| UniProt | Q7ZVX4 , Q6PE19 |
| Localization | Cytoplasm |
| Applications | Western Blot : 0.5-1ug/ml Immunohistochemistry (FFPE) : 2-5ug/ml |
| Limitations | This Zebrafish Rab14 antibody is available for research use only. |
|
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The Zebrafish Rab14 antibody targets Rab14, also known as Rab14l in Danio rerio, a member of the Rab family of small GTPases that regulate intracellular membrane trafficking, organelle positioning, and polarized transport during embryonic development. Zebrafish, also referred to as Danio rerio, express rab14 broadly across early embryogenesis, with enriched levels in epithelial tissues, neural precursors, developing somites, and endoderm-derived organs. Rab14 localizes primarily to endosomes, the trans-Golgi network, and recycling pathways, where it governs vesicle budding, cargo sorting, and delivery to specific membrane domains crucial for morphogenesis and cell polarity.
Rab14 belongs to the Ras superfamily of GTP-binding proteins and cycles between an active GTP-bound state and an inactive GDP-bound state, allowing it to function as a molecular switch for membrane-trafficking pathways. In zebrafish embryos, Rab14 supports dynamic membrane rearrangements required for rapid proliferation, axis formation, and tissue organization. A Zebrafish Rab14 antibody is suitable for detecting Rab14 in endosomal and perinuclear regions, providing insight into membrane transport routes that support signaling, nutrient handling, and morphogen distribution during development.
Functionally, Rab14 plays a central role in coordinating endocytic and recycling pathways that regulate cellular communication and surface receptor presentation. Studies in vertebrate systems show Rab14 involvement in apical-basal polarity, epithelial lumen formation, and trafficking of key regulators including tight junction proteins, guidance receptors, and morphogen modulators. In zebrafish, these functions align with Rab14 expression in tissues undergoing rapid morphogenesis, where trafficking precision impacts neural tube patterning, somitogenesis, cardiac chamber development, gut formation, and sensory organ differentiation. Disruption of rab14 can impair membrane recycling efficiency, alter distribution of signaling receptors, and disturb coordinated migration of developing cell populations.
Structurally, zebrafish Rab14 contains conserved GTP-binding domains and switch regions that interact with effector proteins required for vesicle docking, membrane tethering, and cargo selection. Rab14 associates with endosomal membranes through prenylation and interacts with regulators such as GEFs and GAPs that modulate its activity cycle. The zebrafish rab14 gene maps to chromosome 5 and is developmentally regulated by transcriptional programs that control membrane dynamics, epithelial maturation, and intracellular transport balance. Co-localization analyses in developing tissues identify Rab14 in early endosomes, recycling endosomes, and Golgi-associated compartments, overlapping with markers of trafficking regulators and cytoskeletal remodeling machinery.
A Zebrafish Rab14 antibody is suitable for detecting Rab14 in studies focused on membrane trafficking, cell polarity, intracellular transport dynamics, epithelial morphogenesis, and endosomal signaling networks in Danio rerio. Because vesicle traffic influences distribution of morphogens and receptors essential for developmental pathways including Wnt, Fgf, Hedgehog, and Notch, Rab14 serves as an important marker of endosomal regulation and developmental transport fidelity. This antibody is supplied for research use by NSJ Bioreagents.
Optimal dilution of the Zebrafish Rab14 antibody should be determined by the researcher.
E. coli-derived zebrafish Rab14 recombinant protein (amino acids D108-C215) was used as the immunogen for the Zebrafish Rab14 antibody. This antibody will detect Rab14 and Rab14l protein.
After reconstitution, the Zebrafish Rab14 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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