Zebrafish Psmd8 Antibody / 26S proteasome non-ATPase regulatory subunit 8 (RZ1296)

Description

Psmd8 (Proteasome 26S subunit, non-ATPase 8) is an essential component of the 19S regulatory particle of the 26S proteasome, playing a vital role in the ATP-dependent degradation of ubiquitinated proteins. As part of the regulatory complex, Psmd8 is involved in the recognition, unfolding, and translocation of substrates into the 20S catalytic core, where they are degraded into smaller peptides. This process is critical for maintaining cellular homeostasis, regulating the cell cycle, and ensuring the proper turnover of proteins, which helps prevent the accumulation of damaged or misfolded proteins.

In zebrafish, Psmd8 is the ortholog of the human PSMD8 gene, sharing a high degree of sequence conservation and functional similarity. Both the zebrafish and human versions of Psmd8 contain conserved domains required for proteasomal assembly and interaction with other proteasomal subunits, facilitating the proper function of the 26S proteasome complex. The conservation between these species underscores the value of zebrafish as a model organism for studying proteasomal mechanisms and their contribution to human health.

Psmd8 in zebrafish may also have isoforms due to alternative splicing events. These isoforms, while not fully characterized, could exhibit tissue-specific expression or distinct functional roles in different stages of development. Variations in isoforms can allow for the fine-tuning of proteasomal activity in response to different cellular environments, including stress or differentiation signals. The presence of multiple isoforms enhances the adaptability of the proteasome in maintaining cellular balance across various tissues.

In zebrafish, Psmd8 is expressed in several tissues, particularly those involved in high protein turnover and cellular division, such as the brain, liver, and kidney. This broad expression pattern highlights its essential role in controlling the degradation of regulatory proteins and supporting cellular processes like growth, stress responses, and apoptosis.

The functional conservation of Psmd8 between zebrafish and humans makes it an excellent model for understanding the molecular mechanisms underlying proteasomal dysfunctions in humans. Alterations in PSMD8 function have been implicated in various diseases, including neurodegenerative disorders and cancers, where impaired proteasomal degradation leads to the accumulation of toxic proteins. Zebrafish Psmd8 offers a valuable tool for studying these diseases and exploring potential therapeutic strategies aimed at modulating proteasomal activity to treat proteostasis-related disorders.

Application Notes

Optimal dilution of the Zebrafish Psmd8 antibody should be determined by the researcher.

Immunogen

E. coli-derived zebrafish Psmd8 recombinant protein (amino acids M1-V267) was used as the immunogen for the Zebrafish Psmd8 antibody.

Storage

After reconstitution, the Zebrafish Psmd8 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.